101 - Pichia membranifaciens

Strain Information:

NCYC Number:101
Name:Pichia membranifaciens
Depositor:A. Chaston Chapman
Deposit Name:Saccharomyces fragilis
Deposit Month:February
Deposit Year:1921
Equivalent Strain Designations:ATCC 10022; CBS 6432; CCRC 21628; NCTC 2303; NRRL Y-665
Habitat:Kefir beer
References:Die Mikroorg. d. Gärungs-Industrie, 1918, 276.^Vienne P & Von Stockar U. Alcohol from whey permeate: strain selection, temperature, and medium optimization. Biotechnol. Bioeng. Symp. 13: 421-435, 1983.^Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994.^Vienne P, Von Stockar U. Alcohol from whey permeate: strain selection, temperature, and medium optimization. Biotechnol. Bioeng. Symp. 13: 421-435, 1983. ^

Physical Characteristics:

Optimum Temperature: °
Miniumum Temperature: °
Maximum Temperature: °

Cells:

Shape:Bot
Min Broth Breadth:2
Max Broth Breadth:4
Min Broth Length:4
Max Broth Length:16
Min Agar Breadth:2
Max Agar Breadth:3
Min Agar Length:3
Max Agar Length:255
Arrangement:Chains
Colour on Agar:Brown
Surface on Agar:Dull
Texture on Agar:Wrinkled
Deposit in Broth:Unknown
Ring in Broth:Unknown
Ring Colour:Unknown
Pellicle in Broth:Thick
Pellicle Appearance:Wrinkled
Pellicle Habitat:Climbing

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Well formed
Pseudomycelium Branch:Irregularly branched
Pseudomycelium Form:Unknown
Blastospores:Few
Blastospore Shape:Oval
Blastospore Location:Verticils
Blastospore Habit:Unknown
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Present
Ascospore Shape:Round
Ascospore Wall:Smooth
Ascospore No Per Ascus:4
Ascus Shape:Oval
Conjugation:Absent
Teliospores:Absent
Teliospore Shape: 

Miscellaneous:

Assay:Unknown
Salt Tolerant:10%
Killer:No
Plasmid:Unknown

Semi-Anaerobic Fermentation:

Glucose:-
Galactose:-
Sucrose:-
Maltose:-
Cellobiose:Unknown
Trehalose:Unknown
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:Unknown
Inulin:Unknown
Soluble Starch:-
Xylose:Unknown
A M D Glucoside:Unknown

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:-
Sorbose:-
Sucrose:-
Maltose:-
Cellobiose:-
Trehalose:-
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:-
Inulin:-
Soluble Starch:-
Xylose:-
L Arabinose:-
D Arabinose:-
Ribose:-
Rhamnose:-
Ethanol:-
Glycerol:+
Erythritol:-
Ribitol:-
Galactitol:-
Mannitol:-
Sorbitol:-
A M D Glucoside:Unknown
Salicin:-
Lactic Acid:Unknown
Succinic Acid:Unknown
Citric Acid:Unknown
Inositol:-
Gluconolactone:Unknown
Glucosamine:Unknown
Methanol:Unknown
Xylitol:Unknown

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:-
KNO3:+
Ethylamine:+
Cadaverine:Unknown
Lysine: 

Other:

Vitamin Free Growth:Unknown
Cyclohex 100ppm:Unknown
Cyclohex 1000ppm:Unknown
50% Glucose Growth:Unknown
60% Glucose Growth:Unknown
Lipolytic:-
Acid Production�:Unknown
37c Growth:-
40c Growth: 
Arbutin Hydrolysis:-
Urease Activity:Unknown
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:Produces ethanol from whey permeate (Vienne P & Von Stockar U, 1983). ^Produces glycerol from whey permeate (Rapin JD, et al., 1994).^Degrades whey permeate (Vienne P, Von Stockar U, 1983; Rapin JD, et al., 1994).^Produces UDP-glucose 4-epimerase (udp-glucose 4-epimerase (galactowaldenase).^Produces ethyl alcohol (ethanol) (Vienne P & Von Stockar U, 1983). ^Produces glycerol (glycerin; glycerine) (Rapin JD, et al., 1994).^

Sequence Data:

Sequence Data:Data not available.