1541 - Pichia stipitis

Strain Information:

NCYC Number:1541
Name:Pichia stipitis
Depositor:CBS
Deposit Name:Pichia stipitis
Deposit Month: 
Deposit Year:1984
Equivalent Strain Designations:ATCC 58376, CBS 5773, CCRC 21775, DBVPG 6264, LY 1321, NRRL Y-7124.^
Habitat:Insect larva, on fruit tree, Lyon, Rhône, France.
References:Pignal, Bull.Mens.Soc.Linn.Lyon 36: 163-168, 1967.^Vaughan Martini, Ann.Fac.Agric.Univ.Perugia 38: 331-335, 1984.^Billon-Grand, Mycotaxon 35: 201-204, 1989 (CoQ).^Kurtzman, A.v.Leeuwenhoek 57: 215-222, 1990 (DNA).^Rizzi et al., Appl.Microbiol.Biotechnol. 29: 148-154, 1988.^Björling,T.,Lindman,B. Evaluation of xylose-fermenting yeasts for ethanol production from spent sulfite liquor.Enzyme and Microbial Technology. 11: 240-246, 1989 (Xylose).^Dellweg,H et al. Xylose fermentation by yeasts 3. Comparison of Pachysolen tannophilus and Pichia stipitis.Biotechnology Letters. 6:395-400, 1984 (Xylose).^Lee,H. et al. Utilization of xylan by yeasts and its conversion to ethanol by Pichia stipitis strain.Applied and Environmental Microbiology.52:320-324, 1986 (Xylan).

Physical Characteristics:

Optimum Temperature:0°
Miniumum Temperature:0°
Maximum Temperature:0°

Cells:

Shape:Oval
Min Broth Breadth:2
Max Broth Breadth:4
Min Broth Length:2
Max Broth Length:5
Min Agar Breadth:2
Max Agar Breadth:5
Min Agar Length:3
Max Agar Length:5
Arrangement:Single
Colour on Agar:Cream
Surface on Agar:Slightly shiny
Texture on Agar:Smooth
Deposit in Broth:Non-Flocculent
Ring in Broth:Incomplete
Ring Colour:White
Pellicle in Broth:Absent
Pellicle Appearance: 
Pellicle Habitat: 

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Well formed
Pseudomycelium Branch:Irregularly branched
Pseudomycelium Form: 
Blastospores:Few
Blastospore Shape:Oval
Blastospore Location:Dispersed
Blastospore Habit:Single
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Absent
Ascospore Shape: 
Ascospore Wall: 
Ascospore No Per Ascus: 
Ascus Shape: 
Conjugation:Absent
Teliospores:Absent
Teliospore Shape: 

Miscellaneous:

Assay:No
Salt Tolerant:-
Killer:No
Plasmid:Unknown

Semi-Anaerobic Fermentation:

Glucose:Weak/Latent
Galactose:Weak/Latent
Sucrose:-
Maltose:-
Cellobiose:-
Trehalose:Weak/Latent
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:Weak/Latent
Inulin:-
Soluble Starch:Weak/Latent
Xylose:Unknown
A M D Glucoside:Weak/Latent

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:+
Sorbose:-
Sucrose:+
Maltose:+
Cellobiose:+
Trehalose:+
Lactose:+
Melibiose:-
Raffinose:-
Melizitose:+
Inulin:-
Soluble Starch:+
Xylose:+
L Arabinose:-
D Arabinose:Weak/Latent
Ribose:+
Rhamnose:+
Ethanol:+
Glycerol:+
Erythritol:+
Ribitol:+
Galactitol:-
Mannitol:+
Sorbitol:+
A M D Glucoside:+
Salicin:+
Lactic Acid:-
Succinic Acid:+
Citric Acid:+
Inositol:-
Gluconolactone:+
Glucosamine:Weak/Latent
Methanol:-
Xylitol:Unknown

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:+
KNO3:-
Ethylamine:+
Cadaverine:+
Lysine: 

Other:

Vitamin Free Growth:-
Cyclohex 100ppm:-
Cyclohex 1000ppm:-
50% Glucose Growth:-
60% Glucose Growth:-
Lipolytic:-
Acid Production�:-
37c Growth:-
40c Growth: 
Arbutin Hydrolysis:Weak/Latent
Urease Activity:-
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:Production of ethanol from xylose. Convertion xylan into ethanol (Lee,H. et al. 1986)^

Sequence Data:

Sequence Data:Data not available.