175 - Pichia pastoris

Strain Information:

NCYC Number:175
Name:Pichia pastoris
Depositor:A. Guilliermond
Deposit Name:Saccharomyces pastori / Zygosaccharomyces pastori
Deposit Month: 
Deposit Year:1925
Equivalent Strain Designations:ATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.
Habitat:Exudate of Chestnut tree, near Lyon, France.
References:Guilliermond, CR.Hebd. Séances Mem.Soc.Biol. 82: 466-470, 1919.^Nakase & Komagata, J.Gen.Appl.Microbiol. 16: 511-521, 1970 (GC).^Bicknell & Douglas, J.Bacteriol. 101: 505, 1970 (GC).^Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ).^Duff SJ, Murray WD. Production and application of methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 31: 44-49, 1988a.^Duff SJ, Murray WD. Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde. Biotechnol. Bioeng. 31: 790-795, 1988b.^Duff SJ, Murray WD. Production of flavor aldehydes using nongrowing whole cells of Pichia pastoris. Ann. N.Y. Acad. Sci. 542: 428-433, 1988c.^Murray WD, et al. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32: 95-100, 1989.^Duff SJ, Murray WD. Oxidation of benzyl alcohol by whole cells of Pichia pastoris and by alcohol oxidase in aqueous and nonaqueous reaction media. Biotechnol. Bioeng. 34: 153-159, 1989.^Murray WD, et al. Catabolite inactivation in the methylotrophic yeast Pichia pastoris. Appl. Environ. Microbiol. 56: 2378-2383, 1990.^Wegner EH. Treatment of make-up water for use in a fermentation process for growth of yeast cells requiring growth factors. US Patent 4,226,939 dated Oct 7 1980^Murray WD, et al. Production of natural flavor aldehydes from natural source primary alcohols C2-C7. US Patent 4,871,669 dated Oct 3 1989^Phaff HJ. A proposal for amendment of the diagnosis of the genus Pichia Hansen. Antonie van Leeuwenhoek 22: 113-116, 1956.^Appl. Microbiol. Biotechnol. 36: 82-86, 1991.^Yamada Y, et al. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci. Biotechnol. Biochem. 59: 439-444, 1995. ^Enzyme Microb. Technol. 11: 770-775, 1989.^Agric. Biol. Chem. 54: 1879-1882, 1990.^Duff SJB, Murray WD. Metabolism of hydrogen peroxide by Pichia pastoris. Agric. Biol. Chem. 54: 1967-1974, 1990.^^

Physical Characteristics:

Optimum Temperature: °
Miniumum Temperature: °
Maximum Temperature: °

Cells:

Shape:Oval
Min Broth Breadth:3
Max Broth Breadth:5
Min Broth Length:3
Max Broth Length:6
Min Agar Breadth:2
Max Agar Breadth:5
Min Agar Length:3
Max Agar Length:6
Arrangement:Pairs
Colour on Agar:Cream
Surface on Agar:Shiny
Texture on Agar:Smooth
Deposit in Broth:Non-Flocculent
Ring in Broth:Incomplete
Ring Colour:White
Pellicle in Broth:Absent
Pellicle Appearance:N/A
Pellicle Habitat:N/A

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Well formed
Pseudomycelium Branch:Irregularly branched
Pseudomycelium Form:Mycocandida
Blastospores:Absent
Blastospore Shape:N/A
Blastospore Location:N/A
Blastospore Habit:N/A
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Present
Ascospore Shape:Hat
Ascospore Wall:Unknown
Ascospore No Per Ascus:4
Ascus Shape:Unknown
Conjugation:Present
Teliospores:Absent
Teliospore Shape:N/A

Miscellaneous:

Assay:Unknown
Salt Tolerant:Unknown
Killer:No
Plasmid:No

Semi-Anaerobic Fermentation:

Glucose:+
Galactose:-
Sucrose:-
Maltose:-
Cellobiose:-
Trehalose:-
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:-
Inulin:-
Soluble Starch:-
Xylose:Unknown
A M D Glucoside:-

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:-
Sorbose:-
Sucrose:-
Maltose:-
Cellobiose:-
Trehalose:+
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:-
Inulin:-
Soluble Starch:-
Xylose:-
L Arabinose:-
D Arabinose:-
Ribose:-
Rhamnose:+
Ethanol:+
Glycerol:+
Erythritol:-
Ribitol:-
Galactitol:-
Mannitol:+
Sorbitol:Weak/Latent
A M D Glucoside:-
Salicin:-
Lactic Acid:Weak/Latent
Succinic Acid:+
Citric Acid:+
Inositol:-
Gluconolactone:-
Glucosamine:-
Methanol:+
Xylitol:-

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:+
KNO3:-
Ethylamine:+
Cadaverine:+
Lysine:Unknown

Other:

Vitamin Free Growth:Weak/Latent
Cyclohex 100ppm:+
Cyclohex 1000ppm:+
50% Glucose Growth:-
60% Glucose Growth:-
Lipolytic:-
Acid Production�:Weak/Latent
37c Growth:-
40c Growth:Unknown
Arbutin Hydrolysis:-
Urease Activity:-
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:Production of yeast protein from hydrocarbons (US Patent 4,226,939).^Produces natural flavor aldehydes (Murray WD, 1989).^Converts ethanol to acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).^Degrades ethyl alcohol (ethanol) (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).^Degrades hydrogen peroxide (Duff SJB, Murray WD, 1990).^Produces acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989).^Produces alcohol oxidase (Murray WD, et al, 1989; Murray WD, et al, 1990; Agric. Biol. Chem. 54: 1879-1882, 1990).

Sequence Data:

Sequence Data:Data not available.