240 - Saccharomyces cerevisiae

Strain Information:

NCYC Number:	240
Name:	Saccharomyces cerevisiae
Depositor:	B.M. Brown
Deposit Name:	Saccharomyces cerevisiae
Deposit Month:	June
Deposit Year:	1951
Equivalent Strain Designations:	Whitbread Taylor Walker Yeast SC12. ATCC 44953, MUCL 30938.
Habitat:	Ale production yeast.
References:	Woods LF , Wiseman A . Benzo(a) pyrene hydroxylase from Saccharomyces cerevisiae. Substance binding, spectral and kinetic data. Biochim. Biophys. Acta 613: 52-61, 1980.^Restriction analysis of 2ľ plasmid, J.Inst.Brew., 1982, 88, 7-10.

Physical Characteristics:

Optimum Temperature:	30°
Miniumum Temperature:	6.6°
Maximum Temperature:	35°

Cells:

Shape:	Round-oval
Min Broth Breadth:	5
Max Broth Breadth:	8
Min Broth Length:	8
Max Broth Length:	10
Min Agar Breadth:	4
Max Agar Breadth:	6
Min Agar Length:	6
Max Agar Length:	10
Arrangement:	Pairs
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Smooth
Deposit in Broth:	Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Absent
Pellicle Appearance:	N/A
Pellicle Habitat:	N/A

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Absent
Pseudomycelium Branch:	N/A
Pseudomycelium Form:	N/A
Blastospores:	Absent
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habit:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Absent
Ascospore Shape:	N/A
Ascospore Wall:	N/A
Ascospore No Per Ascus:	N/A
Ascus Shape:	N/A
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Miscellaneous:

Assay:	No
Salt Tolerant:	-
Killer:	No
Plasmid:	Yes

Semi-Anaerobic Fermentation:

Glucose:	+
Galactose:	+
Sucrose:	+
Maltose:	+
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	+
Melizitose:	+
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	+

Aerobic Utilistaion and Growth:

Glucose:	+
Galactose:	+
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	-
Trehalose:	+
Lactose:	-
Melibiose:	-
Raffinose:	Weak/Latent
Melizitose:	Weak/Latent
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	Weak/Latent
Glycerol:	-
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	Weak/Latent
Salicin:	-
Lactic Acid:	-
Succinic Acid:	-
Citric Acid:	-
Inositol:	-
Gluconolactone:	-
Glucosamine:	-
Methanol:	-
Xylitol:	-

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:	+
KNO3:	-
Ethylamine:	-
Cadaverine:	-
Lysine:	Unknown

Other:

Vitamin Free Growth:	-
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Productionďż˝:	-
37c Growth:	+
40c Growth:	Unknown
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Brewing Strain Information:

Head:	intermediate
Deposit:	8-15mm
Attenuation:	1.010-1.012
Fermentation Rate:	8-10°
Clarity:	10-20
Flocculence:
Date of Deposit:
Description:

Information:

Information:	Produces benzopyrene hydroxylase [benzo(a)pyrene hydroxylase] ^O3, DMS 63 ľg/l, high pyruvic, low acetic, S.G. 6.9, pH 3.91 used in BRF brewery where it has proved a stable yeast. Fermentation stops at 1012 in batch fermentation; no rising gas bubbles to keep it in suspension. Produces very rough variants which can form 20% of the population and do not assimilate maltotriose. Maltotriose is fermented relatively slowly by the normal strain. (See NCYC 753 & 754).

Produces benzopyrene hydroxylase [benzo(a)pyrene hydroxylase] ^O3, DMS 63 ľg/l, high pyruvic, low acetic, S.G. 6.9, pH 3.91 used in BRF brewery where it has proved a stable yeast. Fermentation stops at 1012 in batch fermentation; no rising gas bubbles to keep it in suspension. Produces very rough variants which can form 20% of the population and do not assimilate maltotriose. Maltotriose is fermented relatively slowly by the normal strain. (See NCYC 753 & 754).

Sequence Data:

Sequence Data:	Data not available.