416 - Kluyveromyces lactis

Strain Information:

NCYC Number:416
Name:Kluyveromyces lactis
Depositor:CBS
Deposit Name:Saccharomyces lactis
Deposit Month: 
Deposit Year:1955
Equivalent Strain Designations:ATCC 56498, CBS 683, DBVPG 6305, DSM 4394, IFO 1090, NRRL Y-8279, UCD 71-59.
Habitat:Gassy cheese.
References:Allen & Thornley Ann.Appl.Biol. 16: 578, 1929.^Steensma, Curr.Genet. 14: 311-317.^GenLit: Coria et al, A.v.Leeuwenhoek 58: 227-234, 1990 (mtDNA).^Cong et al., Microbiology 140: 1327-1335, 1994 (plasmid).^Vaughan-Martini, A. & Martini, A. 1987. Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation. International Journal of Systematic Bacteriology, 37, 380-385.^Grieve PA, et al.. Partial characterization of cheese-ripening proteinases produced by the yeast Kluyveromyces lactis. J. Dairy Res. 50: 469-480, 1983.^Inchaurrondo VA, et al.. Yeast growth and -galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994.^Blondeau K, et al. Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture. Yeast 10: 1297-1303, 1994.^Appl. Microbiol. Biotechnol. 40: 306-308, 1993.^Biotechnol. Tech. 7: 609-614, 1993.^

Physical Characteristics:

Optimum Temperature: °
Miniumum Temperature: °
Maximum Temperature: °

Cells:

Shape:Long-oval
Min Broth Breadth:2
Max Broth Breadth:4
Min Broth Length:3
Max Broth Length:7
Min Agar Breadth:5
Max Agar Breadth:7
Min Agar Length:6
Max Agar Length:8
Arrangement:Unknown
Colour on Agar:Cream
Surface on Agar:Shiny
Texture on Agar:Smooth
Deposit in Broth:Flocculent
Ring in Broth:Absent
Ring Colour:Unknown
Pellicle in Broth:Thin
Pellicle Appearance:Milky
Pellicle Habitat:Climbing

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Well formed
Pseudomycelium Branch:Irregularly branched
Pseudomycelium Form:Mycocandida
Blastospores:Absent
Blastospore Shape:N/A
Blastospore Location:N/A
Blastospore Habit:N/A
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Absent
Ascospore Shape:N/A
Ascospore Wall:N/A
Ascospore No Per Ascus:N/A
Ascus Shape:N/A
Conjugation:Absent
Teliospores:Absent
Teliospore Shape:Unknown

Miscellaneous:

Assay:No
Salt Tolerant:Unknown
Killer:Unknown
Plasmid:No

Semi-Anaerobic Fermentation:

Glucose:+
Galactose:Weak/Latent
Sucrose:Weak/Latent
Maltose:-
Cellobiose:-
Trehalose:-
Lactose:Weak/Latent
Melibiose:-
Raffinose:Weak/Latent
Melizitose:-
Inulin:-
Soluble Starch:-
Xylose:Unknown
A M D Glucoside:-

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:+
Sorbose:-
Sucrose:+
Maltose:+
Cellobiose:+
Trehalose:+
Lactose:+
Melibiose:-
Raffinose:+
Melizitose:+
Inulin:-
Soluble Starch:-
Xylose:Weak/Latent
L Arabinose:-
D Arabinose:-
Ribose:-
Rhamnose:-
Ethanol:+
Glycerol:+
Erythritol:-
Ribitol:-
Galactitol:-
Mannitol:+
Sorbitol:+
A M D Glucoside:+
Salicin:+
Lactic Acid:+
Succinic Acid:+
Citric Acid:-
Inositol:-
Gluconolactone:-
Glucosamine:Weak/Latent
Methanol:-
Xylitol:+

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:+
KNO3:-
Ethylamine:+
Cadaverine:+
Lysine:Unknown

Other:

Vitamin Free Growth:-
Cyclohex 100ppm:+
Cyclohex 1000ppm:-
50% Glucose Growth:-
60% Glucose Growth:Unknown
Lipolytic:-
Acid Production�:-
37c Growth:-
40c Growth:Unknown
Arbutin Hydrolysis:+
Urease Activity:-
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:MT alpha.^Campbell serotype C.^Produces beta-galactosidase (beta-D-galactosidase) (Grieve PA, et al., 1983; Inchaurrondo VA, 1994).^Production of cheese-ripening proteinases (Grieve PA, et al., 1983).^Transformation host (Blondeau K, 1994; Appl. Microbiol. Biotechnol. 40: 306-308, 1993; Biotechnol. Tech. 7: 609-614, 1993).^Disinfectant testing.^

Sequence Data:

Sequence Data:Data not available.