443 - Pichia guilliermondii

Strain Information:

NCYC Number:443
Name:Pichia guilliermondii
Depositor:L.J. Wickerham, NRRL, USA.
Deposit Name:Candida guilliermondii
Deposit Month:March
Deposit Year:1955
Equivalent Strain Designations:ATCC 46036, CCRC 21697, CCRC 22093, DBVPG 6571, IFO 10106, NRRL Y-2075.
Habitat:Insect frass on Ulmus americana (elm tree), Peoria, Illinois, USA.
References:Fuson et al., Int.J.Syst.Bacteriol. 30: 217-219, 1980 (DNA)^Wickerham LJ . Validation of the species Pichia guilliermondii. J. Bacteriol. 92: 1269, 1966. ^Wickerham LJ , Burton KA . A clarification of the relationship of Candida guilliermondii to other yeasts by a study of their mating types. J. Bacteriol. 68: 594-597, 1954. ^Saha BC , Bothast RJ . Production of L-arabitol for L-arabinose by Candida entomaea and Pichia guilliermondii. Appl. Microbiol. Biotechnol. 45: 299-306, 1996. ^Billon-Grand G . A new ascosporogenous yeast genus: Yamadazyma gen. nov.. Mycotaxon 35: 201-204, 1989. ^Church FC , et al.. Isolation and characterization of alpha-galactosidase from Pichia guilliermondii. Dev. Ind. Microbiol. 21: 339-348, 1980. ^Church FC Jr, Meyers SP . Alpha-galactosidase from Pichia guilliermondii. Mycologia 72: 279-287, 1980. ^Hou CT . pH dependence and thermostability of lipases from cultures from the ARS Culture Collection. J. Ind. Microbiol. 13: 242-248, 1994.

Physical Characteristics:

Optimum Temperature: °
Miniumum Temperature: °
Maximum Temperature: °

Cells:

Shape:Round-oval
Min Broth Breadth:1
Max Broth Breadth:4
Min Broth Length:3
Max Broth Length:10
Min Agar Breadth:2
Max Agar Breadth:3
Min Agar Length:2
Max Agar Length:12
Arrangement:Pairs
Colour on Agar:Cream
Surface on Agar:Shiny
Texture on Agar:Smooth
Deposit in Broth:Non-Flocculent
Ring in Broth:Complete
Ring Colour:White
Pellicle in Broth:Thin
Pellicle Appearance:Wrinkled
Pellicle Habitat:Unknown

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Well formed
Pseudomycelium Branch:Unknown
Pseudomycelium Form:Unknown
Blastospores:Unknown
Blastospore Shape:Oval
Blastospore Location:Unknown
Blastospore Habit:Unknown
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Absent
Ascospore Shape:N/A
Ascospore Wall:N/A
Ascospore No Per Ascus:N/A
Ascus Shape:N/A
Conjugation:Absent
Teliospores:Absent
Teliospore Shape:N/A

Miscellaneous:

Assay:Unknown
Salt Tolerant:Unknown
Killer:Unknown
Plasmid:Unknown

Semi-Anaerobic Fermentation:

Glucose:+
Galactose:Weak/Latent
Sucrose:+
Maltose:-
Cellobiose:Unknown
Trehalose:Unknown
Lactose:-
Melibiose:-
Raffinose:+
Melizitose:Unknown
Inulin:Unknown
Soluble Starch:Unknown
Xylose:Unknown
A M D Glucoside:Unknown

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:+
Sorbose:Unknown
Sucrose:+
Maltose:+
Cellobiose:+
Trehalose:Unknown
Lactose:Unknown
Melibiose:+
Raffinose:Unknown
Melizitose:Unknown
Inulin:Unknown
Soluble Starch:Unknown
Xylose:+
L Arabinose:+
D Arabinose:+
Ribose:Weak/Latent
Rhamnose:Unknown
Ethanol:Unknown
Glycerol:Unknown
Erythritol:-
Ribitol:+
Galactitol:Unknown
Mannitol:+
Sorbitol:+
A M D Glucoside:Unknown
Salicin:Unknown
Lactic Acid:Unknown
Succinic Acid:Unknown
Citric Acid:Unknown
Inositol:-
Gluconolactone:Unknown
Glucosamine:Unknown
Methanol:Unknown
Xylitol:Unknown

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:+
KNO3:-
Ethylamine:Unknown
Cadaverine:Unknown
Lysine:Unknown

Other:

Vitamin Free Growth:+
Cyclohex 100ppm:Unknown
Cyclohex 1000ppm:Unknown
50% Glucose Growth:Unknown
60% Glucose Growth:Unknown
Lipolytic:-
Acid Production�:Unknown
37c Growth:Unknown
40c Growth:Unknown
Arbutin Hydrolysis:+
Urease Activity:Unknown
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:Produces lipases active at pH 9.0 at 60C (Hou CT , 1994).^Ferments arabinose [L-arabinose] (Saha BC , Bothast RJ , 1996).^Produces L-arabitol (Saha BC , Bothast RJ , 1996).^Produces alpha-galactosidase (Church FC , et al, 1980, Church FC Jr, Meyers SP , 1980).^Produces lipase (Hou CT , 1994.)^Opposite mating type to NCYC 441.

Sequence Data:

Sequence Data:Data not available.