614 - Pachysolen tannophilus

Strain Information:

NCYC Number:614
Name:Pachysolen tannophilus
Depositor:CBS
Deposit Name:Pachysolen tannophilus
Deposit Month:April
Deposit Year:1960
Equivalent Strain Designations:ATCC 32691, CBS 4044, CCRC 20329, DBVPG 6666, IMI 344640, MUCL 31901, NRRL Y-2460
Habitat:Extracts of concentrated tanning fluid from Castanea vesca (Chestnut), Germany.
References:Bolen PL , Detroy RW . Induction of NADPH-linked D-xylose reductase and NAD-linked xylitol dehydrogenase activities in Pachysolen tannophilus by D-xylose, L-arabinose, or D-galactose. Biotechnol. Bioeng. 26: 302-307, 1985. ^Ditzelmuller G , et al.. Xylitol dehydrogenase from Pachysolen tannophilus. FEMS Microbiol. Lett. 25: 195-198, 1984. ^Bolen PL , et al.. Affinity purifications of aldose reductase and xylitol dehydrogenase from the xylose-fermenting yeast Pachysolen tannophilus. Appl. Environ. Microbiol. 52: 660-664, 1986. ^Rokem JS , Zomer E . Scale up of the production of xylitol dehydrogenase and alcohol dehydrogenase from Pachysolen tannophilus. Appl. Microbiol. Biotechnol. 26: 231-233, 1987. ^Kurtzman CP , et al.. Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. US Patent 4,359,534 dated Nov 16 1982 ^Jeffries TW . Process for enhanced fermentation of xylose to ethanol. US Patent 4,663,284 dated May 5 1987 ^Mahmourides G , et al.. Ethanol accumulation in cultures of Pachysolen tannophilus on D-xylose is associated with a transition to a state of low oxygen consumption. Bio-Technology 3: 59-62, 1985. ^Boidin J , Adzet JM . Deux curieuses levures isolees d'extraits tannants d'origine vegetale: Pachysolen (nov. gen.) tannophilus nov. sp. et P. pelliculatus nov. sp.. Bull. Soc. Mycol. Fr. 73: 331, 1957. ^Kavanagh K , Whittaker PA . Application of the Melle-Boinot process to the fermentation of xylose by Pachysolen tannophilus. Appl. Microbiol. Biotechnol. 42: 28-31, 1994. ^Vanetti MC , Aquarone E . Riboflavin excretion by Pachysolen tannophilus grown in synthetic medium supplemented with D-xylose. World J. Microbiol. Biotechnol. 8: 190-191, 1992. ^Soto T , et al. Posttranslational regulatory control of trehalase induced by nutrients, metabolic inhibitors, and physical agents in Pachysolen tannophilus. Fungal Genet. Biol. 20: 143-151, 1996. ^Maleszka R , et al.. Ethanol production from D-galactose and glycerol by Pachysolen tannophilus. Enzyme Microb. Technol. 4: 349-352, 1982. ^Schneider H , et al.. Conversion of D-xylose into ethanol by the yeast Pachysolen tannophilus. Biotechnol. Lett. 3: 89-92, 1981. ^Maleszka R , et al.. Enhanced rate of ethanol production from D-xylose using recycled or immobilized cells of Pachysolen tannophilus. Biotechnol. Lett. 3: 415-420, 1981. ^Ohno N , et al.. Purification and properties of an exo(1>3)-beta-D-glucanase from the culture filtrate of Mucor hiemalis. Carbohydr. Res. 158: 217-226, 1986. ^Kurtzman CP . Biology and physiology of the D-xylose fermenting yeast Pachysolen tannophilus. Adv. Biochem. Eng. Biotechnol. 27: 73-83, 1983. ^Slininger PJ , et al. Conversion of D-xylose to ethanol by the yeast Pachysolen tannophilus. Biotechnol. Bioeng. 24: 371-384, 1982. ^Detroy RW , et al. Bioconversion of wheat straw cellulose/ hemicellulose to ethanol by Saccharomyces uvarum and Pachysolen tannophilus. Biotechnol. Bioeng. 24: 1105-1113, 1982.

Physical Characteristics:

Optimum Temperature: °
Miniumum Temperature: °
Maximum Temperature: °

Cells:

Shape:Round-oval
Min Broth Breadth:4
Max Broth Breadth:6
Min Broth Length:4
Max Broth Length:7
Min Agar Breadth:3
Max Agar Breadth:4
Min Agar Length:3
Max Agar Length:7
Arrangement:Pairs
Colour on Agar:Cream
Surface on Agar:Shiny
Texture on Agar:Smooth
Deposit in Broth:Non-Flocculent
Ring in Broth:Absent
Ring Colour:Unknown
Pellicle in Broth:Absent
Pellicle Appearance:N/A
Pellicle Habitat:N/A

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Absent
Pseudomycelium Branch:N/A
Pseudomycelium Form:N/A
Blastospores:N/A
Blastospore Shape:N/A
Blastospore Location:N/A
Blastospore Habit:N/A
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Absent
Ascospore Shape:N/A
Ascospore Wall:N/A
Ascospore No Per Ascus:N/A
Ascus Shape:N/A
Conjugation:Absent
Teliospores:Absent
Teliospore Shape:N/A

Miscellaneous:

Assay:Unknown
Salt Tolerant:Unknown
Killer:Unknown
Plasmid:Unknown

Semi-Anaerobic Fermentation:

Glucose:+
Galactose:-
Sucrose:-
Maltose:-
Cellobiose:-
Trehalose:-
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:-
Inulin:Unknown
Soluble Starch:Unknown
Xylose:Unknown
A M D Glucoside:-

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:Weak/Latent
Sorbose:Unknown
Sucrose:-
Maltose:-
Cellobiose:+
Trehalose:-
Lactose:-
Melibiose:-
Raffinose:-
Melizitose:-
Inulin:Unknown
Soluble Starch:Unknown
Xylose:Unknown
L Arabinose:Unknown
D Arabinose:Unknown
Ribose:Unknown
Rhamnose:Unknown
Ethanol:Unknown
Glycerol:+
Erythritol:-
Ribitol:Unknown
Galactitol:Unknown
Mannitol:+
Sorbitol:Unknown
A M D Glucoside:Unknown
Salicin:Unknown
Lactic Acid:Unknown
Succinic Acid:Unknown
Citric Acid:Unknown
Inositol:-
Gluconolactone:Unknown
Glucosamine:Unknown
Methanol:-
Xylitol:Unknown

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:Unknown
KNO3:+
Ethylamine:Unknown
Cadaverine:Unknown
Lysine:Unknown

Other:

Vitamin Free Growth:Unknown
Cyclohex 100ppm:Unknown
Cyclohex 1000ppm:Unknown
50% Glucose Growth:Unknown
60% Glucose Growth:Unknown
Lipolytic:-
Acid Production�:Unknown
37c Growth:Unknown
40c Growth:Unknown
Arbutin Hydrolysis:-
Urease Activity:Unknown
Starch Production:-
Acid Tolerant:Unknown

Information:

Information:Degrades cellulose. (Detroy RW , 1992).^Degrades hemicellulose. (Detroy RW , 1992).^Ferments D-galactose. (Maleszka R, 1982).^Ferments glycerol [glycerine]. (Maleszka R, 1982).^Ferments xylose [D-xylose] . Kurtzman CP , et al, 1982; Jeffries TW , 1987; Schneider H , et al, 1981; Maleszka R, 1981; Ohno N , 1986; Kurtzman CP, 1983; Slininger P, 1982).^Produces D-xylulose reductase [xylitol dehydrogenase]. (Bolen PL, 1985; Ditzelmuller G, 1984; Bolen PL, 1986; Rokem JS , Zomer E, 1982).^Produces alcohol dehydrogenase. (Bolen PL , 1986).^Produces aldehyde reductase [xylose reductase]. (Bolen PL , 1985; Bolen PL , 1986).^Produces ethyl alcohol [ethanol] .(Kurtzman CP, 1982; Jeffries TW, 1987; Mahmourides G, 1985; Maleszka R, 1982; Schneider H, 1981; Maleszka R, 1981; Kurtzman C , 1983; Slininger PJ , et al, 1982; Detroy RW, 1982).^Produces riboflavin [vitamin B2] .(Vanetti MC , 1992)^Production of ethanol, US Patent 4,359,534.^Enhanced ethanol tolerance (Kavanagh K, 1994).^Trehalase activity (Soto T, 1996).

Sequence Data:

Sequence Data:Data not available.