874 - Phaffia rhodozyma

Strain Information:

NCYC Number:874
Name:Phaffia rhodozyma
Depositor:M.W.Miller
Deposit Name: 
Deposit Month: 
Deposit Year:1980
Equivalent Strain Designations:ATCC 24202, CBS 5905, CCRC 21346, DSM 5626, DBVPG 7009, IFO 10129, IGC 4172, NRRL Y-10921, UCD 67-210.
Habitat:Exudate of Fagus crenata, Japan.
References:Phaff et al., Proc. IV Int.Ferment.Symp.: Ferment.Technol.Today, p.759-774, 1972.^Kucsera et al., A.v.Leuwenhoek 73: 163-168, 1998 (sporulation).^Medwid, J.Indust.Microbiol.Biotechnol. 21: 221-232, 1998 (polypoidy).^Yamada & Kawasaki, Agric.Biol.Chem. 53: 2845-2846, 1989 (18S rRNA sequences).^Pfeiffer et al., Curr.Genet. 30: 294-297, 1996 (dsRNA virus).^Andrewes et al., Phytochem. 15: 1003-1007, 1976; Andrewes & Starr, Phytochem. 15: 1009-1101, 1976; Johnson & Lewis, J.Gen.Microbiol. 115: 173-183, 1979 AppLit: Johnson et al.^Johnson EA , et al. Processes for in vivo production of astaxanthin and Phaffia rhodozyma yeast of enhanced astaxanthin content. US Patent 5,356,809 dated Oct 18 1994.^Golubev WI . Perfect state of Rhodomyces dendrorhous (Phaffia rhodozyma). Yeast 11: 101-110, 1995. ^Miller MW , et al. Phaffia, a new yeast genus in the Deuteromycotina (Blastomycetes). Int. J. Syst. Bacteriol. 26: 286-291, 1976.^Biotechnol. Lett. 17: 575-578, 1995.^Hayman GT , et al. Production of carotenoids by Phaffia rhodozyma grown on media composed of corn wet-milling co-products. J. Ind. Microbiol. 14: 389-395, 1995.^Tilser I , et al. Effect of supplementation of University of Wisconsin solution with glycoproteins from psychrophilic strains of yeast on hypothermic liver storage of rats. Cryobiology 33: 347-353, 1996. ^Breierova E . Cryoprotection of psychrophilic yeast species- by the use of additives with croprotective media. Cryo-Lett. 15: 191-197, 1994.^Fell JW , Blatt GM . Separation of strains of the yeasts Xanthophyllomyces dendrorhous and Phaffia rhodozyma based on rDNA IGS and ITS sequence analysis. J. Ind. Microbiol. Biotechnol. 23: 677-681, 1999. ^Fell JW , et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. ^^^

Physical Characteristics:

Optimum Temperature:23°
Miniumum Temperature:5°
Maximum Temperature:26°

Cells:

Shape:Round-oval
Min Broth Breadth:3
Max Broth Breadth:5
Min Broth Length:4
Max Broth Length:9
Min Agar Breadth:5
Max Agar Breadth:7
Min Agar Length:7
Max Agar Length:12
Arrangement:Pairs
Colour on Agar:Pink
Surface on Agar:Shiny
Texture on Agar:Smooth
Deposit in Broth:Non-Flocculent
Ring in Broth:Absent
Ring Colour:Unknown
Pellicle in Broth:Absent
Pellicle Appearance:N/A
Pellicle Habitat:N/A

Cell Division:

Budding:Multipolar
Fission:Absent

Filamentous Growth:

Pseudomycelium:Absent
Pseudomycelium Branch:N/A
Pseudomycelium Form:N/A
Blastospores:Absent
Blastospore Shape:N/A
Blastospore Location:N/A
Blastospore Habit:N/A
True Mycelium:Absent
Clamp Connections:Absent

Asexual Spores:

Ballistospores:Absent
Arthrospores:Absent
Endospores:Absent
Chlamydospores:Absent

Sexual Spores:

Ascospores:Absent
Ascospore Shape:N/A
Ascospore Wall:N/A
Ascospore No Per Ascus:N/A
Ascus Shape:N/A
Conjugation:Absent
Teliospores:Absent
Teliospore Shape:N/A

Miscellaneous:

Assay:Unknown
Salt Tolerant:-
Killer:Unknown
Plasmid:No

Semi-Anaerobic Fermentation:

Glucose:+
Galactose:-
Sucrose:Weak/Latent
Maltose:-
Cellobiose:Unknown
Trehalose:Unknown
Lactose:-
Melibiose:-
Raffinose:+
Melizitose:Unknown
Inulin:Unknown
Soluble Starch:-
Xylose:Unknown
A M D Glucoside:Unknown

Aerobic Utilistaion and Growth:

Glucose:+
Galactose:-
Sorbose:-
Sucrose:+
Maltose:+
Cellobiose:+
Trehalose:+
Lactose:-
Melibiose:-
Raffinose:+
Melizitose:+
Inulin:-
Soluble Starch:Weak/Latent
Xylose:Weak/Latent
L Arabinose:+
D Arabinose:-
Ribose:-
Rhamnose:-
Ethanol:-
Glycerol:-
Erythritol:-
Ribitol:-
Galactitol:-
Mannitol:+
Sorbitol:-
A M D Glucoside:Weak/Latent
Salicin:-
Lactic Acid:-
Succinic Acid:-
Citric Acid:Unknown
Inositol:-
Gluconolactone:Unknown
Glucosamine:Unknown
Methanol:Unknown
Xylitol:Unknown

Aerobic Utilistaion and Growth - Sole Sources of Nitrogen:

NH4 2SO4:-
KNO3:-
Ethylamine:-
Cadaverine:Unknown
Lysine:Unknown

Other:

Vitamin Free Growth:-
Cyclohex 100ppm:-
Cyclohex 1000ppm:-
50% Glucose Growth:Unknown
60% Glucose Growth:-
Lipolytic:-
Acid Production�:-
37c Growth:-
40c Growth:Unknown
Arbutin Hydrolysis:-
Urease Activity:Unknown
Starch Production:Unknown
Acid Tolerant:-

Information:

Information:Applications: feed supplement for salmonid fish and crustaceans.^Increased production of carotinoids and trans-astaxanthin by addition of mevalonic acid (Biotechnol. Lett. 17: 575-578, 1995).^Produces astaxanthin (Johnson EA , 1994; Biotechnol. Lett. 17: 575-578, 1995).^Produces carotenoids (Biotechnol. Lett. 17: 575-578, 1995; Hayman GT , et al, 1995).^Produces glycoproteins (Tilser I, 1996).

Sequence Data:

Sequence Data:Data not available.