Research

servicesResearch associated with the National Collection of Yeast Cultures aims to improve techniques to identify and classify yeasts, particularly those associated with brewing and food spoilage. This research has shed new light on yeast evolution and genetic interrelationship, including mechanisms of speciation (Fischer et al 2000, Delneri et al 2003) and has developed a number of novel molecular approaches to identifying and characterising yeast biodiversity (e.g. Naumov et al 2000, Wesselink et al 2002). NCYC research is pre-eminent in areas such as phylogenetic analysis of yeasts using ribosomal RNA gene sequences (e.g. Steels et al 2002) and sub-species discrimination using molecular fingerprinting (e.g. Olver et al 2002).

Yeast has long been acknowledged as the most important organism in biotechnology. Our research has demonstrated that there is considerable value contained in the biodiversity of the yeasts that the collection comprises. Such diversity can be of considerable value to a variety of industry sectors including food, pharmaceuticals, fine chemicals, and others. The diversity that has been collected in the NCYC and the know-how and skills of NCYC researchers in understanding and working with the collection is available for contract research. Precision characterisation at the most detailed molecular level makes the collection a most attractive source for commercial partners, particularly when linked to other technologies for physical measurement available within IFR e.g. the metabolomics suite.

NCYC Software

TURNIP

Introduction

As part of the Saccharomyces Genome Resequencing Project, the NCYC undertook the analysis of the rDNA reads from the resequenced S. cerevisiae and S. paradoxus strains. As a result of this research, the NCYC will be releasing a software suite called TURNIP comprising a set of Perl scripts to aid detection of partial SNPs (‘pSNP’), SNPs and indels from unassembled regions of a genome. It is suited to relatively short (10kb – 50kb) regions of DNA, and is able to find sites of microheterogeneity that would otherwise be dismissed through lack of awareness of their existence, or as sequencing error. More about TURNIP

Latest Publications

Norwich BioScience Institutes - Publications

Recently Published Papers

Apiotrichum terrigenum sp. nov., a novel soil-associated yeast found in both the UK and mainland Europe. James S. A., Bond C. J., Stanley R., Ravella S. R., Peter G., Dlauchy D., Roberts I. N. (2016) International Journal of Systematic and Evolutionary Microbiology 66 5046-5050

Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate Senatham S., Chamduang T., Kaewchingduang Y., Thammasittirong A., Srisodsuk M., Elliston A., Roberts I. N., Waldron K., Thammasittirong S. N. (2016) SpringerPlus 5 1040

Prevalence and Dynamics of Ribosomal DNA Micro-heterogeneity Are Linked to Population History in Two Contrasting Yeast Species. James S. A., West C., Davey R. P., Dicks J., Roberts I. N. (2016) Scientific reports 6 28555

Prevalence and Dynamics of Ribosomal DNA Micro-heterogeneity Are Linked to Population History in Two Contrasting Yeast Species. James S. A., West C., Davey R. P., Dicks J., Roberts I. N. (2016) Scientific reports 6 28555

Yeast culture collections in the twenty-first century: new opportunities and challenges Boundy-Mills K. L., Glantschnig E., Roberts I. N., Yurkov A., Casaregola S., Daniel H. -. M., Groenewald M., Turchetti B. (2016) Yeast Online n/a

Reconstructing (super)trees from data sets with missing distances: Not all is lost Kettleborough G., Dicks J., Roberts I. N., Huber K. T. (2015) Molecular Biology and Evolution 32 1628-1642

A comparative study on the potential of epiphytic yeasts isolated from tropical fruits to produce flavouring compounds Grondin E., Sing A. S. C., Caro Y., Raherimandimby M., Randrianierenana A. L., James S. A., Palop C., Francois J. M., Petit T. (2015) International Journal of Food Microbiology 203 101-108

Effect of steam explosion on waste copier paper alone and in a mixed lignocellulosic substrate on saccharification and fermentation Elliston A., Wilson D. R., Collins S. R. A., Roberts I. N., Waldron K. W. (2015) Bioresource Technology 187 136-143

Identification of furfural resistant strains of Saccharomyces cerevisiae and Saccharomyces paradoxus from a collection of environmental and industrial isolates Field S. J., Ryden P., Wilson D., James S. A., Roberts I. N., Richardson D. A., Waldron K. W., Clarke T. A. (2015) Biotechnology for Biofuels 8 33

Kazachstania yasuniensis sp. nov., a novel ascomycetous yeast species found in mainland Ecuador and on the Galapagos James S. A., Carvajal Barriga E. J., Barahona P. P., Palop C., Cross K., Bond C. J., Roberts I. N. (2015) International Journal of Systematic and Evolutionary Microbiology 65 (Pt 4) 1304-1309

Methodology for enabling high-throughput simultaneous saccharification and fermentation (SSF) screening of yeast using solid biomass as a substrate Elliston A., Wood I. P., Soucouri M. J., Tantale R. J., Dicks J., Roberts I. N., Waldron K. W. (2015) Biotechnology for biofuels 8 2

Investigating flavour characteristics of British ale yeasts: techniques, resources and opportunities for innovation Parker N., James S. A., Dicks J., Bond C., Nueno Palop C., White C., Roberts I. N. (2014) Yeast 32 281-287

Ribosomal DNA Sequence Heterogeneity Reflects Intraspecies Phylogenies and Predicts Genome Structure in Two Contrasting Yeast Species West C., James S. A., Davey R. P., Dicks J., Roberts I. N. (2014) Systematic Biology 63 543-554

Wickerhamomyces arborarius sp. nov., a novel ascomycetous yeast species found in arboreal habitats on three different continents James S. A., Carvajal E. J. B., Barahona P. P., Harrington T. C., Lee C. F., Bond C. J., Roberts I. N. (2014) International Journal of Systematic and Evolutionary Microbiology 64 1057-1061

High concentrations of cellulosic ethanol achieved by fed batch semi simultaneous saccharification and fermentation of waste-paper. Elliston A., Collins S. R. A., Wilson D. R., Roberts I. N., Waldron K. W. (2013) Bioresource Technology 134 117-126

Candida ecuadorensis sp. nov., a novel ascomycetous yeast species found in two separate regions of Ecuador James S. A., Carvajal B E. J., Portero B P., Cross K., Bond C. J., Roberts I. N. (2013) International Journal of Systematic and Evolutionary Microbiology 63 393-397

Rapid quantification of reducing sugars in biomass hydrolysates: Improving the speed and precision of the dinitrosalicylic acid assay Wood I. P., Elliston A., Ryden P., Bancroft I., Roberts I. N., Waldron K. W. (2012) Biomass & Bioenergy 44 117-121

Candida theae sp. nov., a new beverage-related member of the Lodderomyces clade Chang C. F., Lin Y. C., Chen S. F., Carvajal E. J., James S. A., Bond C. J., Roberts I. N., Lee C. F. (2012) International Journal of Food Microbiology 153 10-14

Yeasts biodiversity and its significance: Case studies in natural and human-related environments: Ex situ preservation, applications and challenges Carvajal J., Libkind D., Briones A. I., Ubeda J., Portero P., Roberts I. N., James S. A., Morais P. B., Rosa C. A. (2011) 55-86

A geographically diverse collection of Schizosaccharomyces pombe isolates shows limited phenotypic variation but extensive karyotypic diversity Brown W. R. A., Liti G., Rosa C., James S. A., Roberts I. N., Robert V., Jolly N., Tang W., Baumann P., Green C., Schlegel K., Young J., Hirchaud F., Leek S., Thomas G., Blomberg A., Warringer J. (2011) G3: Genes, Genomes, Genetics 1 615-626

 

Site maintained by the Institute of Food Research