Saccharomyces Genome Resequencing Project Strains Information

Saccharomyces Genome Resequencing Project Strains

The Sanger Centre's Saccharomyces Genome Resequencing Project (SGRP) has involved the sequencing of 36 Saccharomyces cerevisiae strains and 35 Saccharomyces paradoxus strains. These strains are available to purchase from the NCYC as NCYC Strain Set 1.

With the exception of the S. cerevisiae laboratory strains S288c and W303, which are both stable haploids, each strain is available as a single spore derivative of the original homothallic parent strain. Please note that these strains retain a functional HO gene (i.e. are HO strains), and are therefore diploid. Stable haploid versions of these strains are currently being created and will, in due course, be made available for purchase.

The SGRP Project is a collaboration between the Sanger Institute, Cambridge and Prof. Ed Louis' group at the Institute of Genetics, University of Nottingham. Further details about the project can be found at:

The Durbin Group
Prof Ed Louis' Group

For further details see Liti et al. (2009). Population genomics of domestic and wild yeasts. Nature 458, 337-341.

Haploid derivatives of Saccharomyces Genome Resequencing Project Strains

View the 77 haploid derivates for Saccharomyces cerevisiae (Set 2)
View the 83 haploid derivates for Saccharomyces paradoxus (Set 3)

Genetically-tractable haploid derivatives of the SGRP set have been created. These strains are available to purchase from the NCYC as NCYC Strain Set 2 (Saccharomyces cerevisiae strains) and NCYC Strain Set 3 (Saccharomyces paradoxus strains).

Three versions of each strain (Haploid Mat a and Mat α and diploid Mat a/α all are ura3 auxotrophs tagged with a 6-bp barcode) are available for most strains (Click here to see which strains are available – link to table). They are stable haploid derivatives and ura3 auxotrophs tagged with a 6-bp barcode, recognized by a restriction enzyme to allow easy identification. The specific barcode can be used to accurately measure the prevalence of different strains during competition experiments. The strains are amenable to a wide variety of genetic experiments and can be easily crossed with each other to create hybrids and segregants, providing a valuable resource for breeding programmes and quantitative genetic studies.

The haploid strains were constructed by Francisco Cubillos, Edward Louis and Gianni Liti at the University of Nottingham.

For further details see Cubillos et al. (2009). Generation of a large set of genetically tractable haploid and diploid Saccharomyces strains. FEMS Yeast Res. 9, 1217-1225