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101 - Pichia membranifaciens

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Strain Information

NCYC Number:	101
Name:	Pichia membranifaciens
Depositor:	A. Chaston Chapman
Deposit Name:	Saccharomyces fragilis
Deposit Month:	February
Deposit Year:	1921
Equivalent Strain Designations:	ATCC 10022; CBS 6432; CCRC 21628; NCTC 2303; NRRL Y-665
Habitat:	Kefir beer
References:	Die Mikroorg. d. Gärungs-Industrie, 1918, 276. Vienne P & Von Stockar U. Alcohol from whey permeate: strain selection, temperature, and medium optimization. Biotechnol. Bioeng. Symp. 13: 421-435, 1983. Rapin JD, et al. Glycerol production by yeast fermentation of whey permeate. Enzyme Microb. Technol. 16: 143-150, 1994. Vienne P, Von Stockar U. Alcohol from whey permeate: strain selection, temperature, and medium optimization. Biotechnol. Bioeng. Symp. 13: 421-435, 1983.
Information:	Produces ethanol from whey permeate (Vienne P & Von Stockar U, 1983). Produces glycerol from whey permeate (Rapin JD, et al., 1994). Degrades whey permeate (Vienne P, Von Stockar U, 1983; Rapin JD, et al., 1994). Produces UDP-glucose 4-epimerase (udp-glucose 4-epimerase (galactowaldenase). Produces ethyl alcohol (ethanol) (Vienne P & Von Stockar U, 1983). Produces glycerol (glycerin; glycerine) (Rapin JD, et al., 1994).

Special Applications

Special Applications:	Production: Ethanol Production: Glycerol

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Bot
Min Broth Breadth:	2
Max Broth Breadth:	4
Min Broth Length:	4
Max Broth Length:	16
Min Agar Breadth:	2
Max Agar Breadth:	3
Min Agar Length:	3
Max Agar Length:	255
Arrangement:	Chains
Colour on Agar:	Brown
Surface on Agar:	Dull
Texture on Agar:	Wrinkled
Deposit in Broth:	Unknown
Ring in Broth:	Unknown
Ring Colour:	Unknown
Pellicle in Broth:	Thick
Pellicle Appearance:	Wrinkled
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:	Unknown
Blastospores:	Few
Blastospore Shape:	Oval
Blastospore Location:	Verticils
Blastospore Habitat:	Unknown
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Round
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	4
Ascus Shape:	Oval
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	Unknown
Salt Tolerant:	10%
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	Unknown
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	Unknown

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	-
Glycerol:	+
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	Unknown
Salicin:	-
Lactic Acid:	Unknown
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	-
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	-
KNO3:	+
Ethylamine:	+
Cadaverine:	Unknown
Lysine:

Vitamin Free Growth:	Unknown
Cyclohex 100ppm:	Unknown
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	Unknown
Lipolytic:	-
Acid Production:	Unknown
37c Growth:	-
40c Growth:
Arbutin Hydrolysis:	-
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown

Sequence Data:	Data not available.