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1519 - Debaryomyces occidentalis var. occidentalis

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Strain Information

NCYC Number:	1519
Name:	Debaryomyces occidentalis var. occidentalis
Depositor:	Dr. W.M. Ingledew
Deposit Name:	Schwanniomyces alluvius, Schwanniomyces occidentalis var. oc
Deposit Month:	January
Deposit Year:	1984
Equivalent Strain Designations:	R69; ATCC 58017.
Habitat:	Genetically defined strain.
References:	Dhawale, M.R. & Ingledew, W.M. 1983. Starch hydrolysis by derepressed mutants of Schwanniomyces castelli. Biotechnology Letters 5, 185-190.
Information:	Strain derived from ATCC26077, CBS 2863. Derepressed for amylases. Produces elevated levels of alpha-amylase and glucoamylase. Produces amylase, alpha 1A; salivary (Amylase, salivary, alpha-1A). Produces glucoamylase.

Special Applications

Special Applications:	Production: Amylase

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):	34.5
Miniumum Temperature(Degrees Celcius):	10
Maximum Temperature (Degrees Celcius):	37

Cells:

Shape:	Round
Min Broth Breadth:	3
Max Broth Breadth:	6
Min Broth Length:	3
Max Broth Length:	6
Min Agar Breadth:	3
Max Agar Breadth:	5
Min Agar Length:	3
Max Agar Length:	5
Arrangement:	Single
Colour on Agar:	Cream
Surface on Agar:	Shiny
Texture on Agar:	Glutinous
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Absent
Ring Colour:	N/A
Pellicle in Broth:	Absent
Pellicle Appearance:	N/A
Pellicle Habitat:	N/A

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:	Unknown
Blastospores:	Absent
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habitat:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Absent
Ascospore Shape:	N/A
Ascospore Wall:	N/A
Ascospore No Per Ascus:	N/A
Ascus Shape:	N/A
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	No
Salt Tolerant:	-
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	Weak/Latent
Galactose:	Weak/Latent
Sucrose:	-
Maltose:	Weak/Latent
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	Weak/Latent
Xylose:	-
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	+
Trehalose:	+
Lactose:	+
Melibiose:	+
Raffinose:	+
Melizitose:	+
Inulin:	Weak/Latent
Soluble Starch:	+
Xylose:	+
L Arabinose:	Weak/Latent
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	+
Erythritol:	-
Ribitol:	+
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	+
Salicin:	+
Lactic Acid:	-
Succinic Acid:	+
Citric Acid:	-
Inositol:	-
Gluconolactone:	+
Glucosamine:	-
Methanol:	-
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:	Unknown

Vitamin Free Growth:	Weak/Latent
Cyclohex 100ppm:	+
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	-
37c Growth:	+
40c Growth:	Unknown
Arbutin Hydrolysis:	+
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	Unknown

Sequence Data:	Data not available.