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1541 - Pichia stipitis

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Strain Information

This is the Pichia stipitis Type Strain

This strain is recommended for studies involving Pichia stipitis

NCYC Number:	1541
Name:	Pichia stipitis
Depositor:	CBS
Deposit Name:	Pichia stipitis
Deposit Month:
Deposit Year:	1984
Equivalent Strain Designations:	ATCC 58376, CBS 5773, CCRC 21775, DBVPG 6264, LY 1321, NRRL Y-7124.^
Habitat:	Insect larva, on fruit tree, Lyon, Rhône, France.
References:	Pignal, Bull.Mens.Soc.Linn.Lyon 36: 163-168, 1967. Vaughan Martini, Ann.Fac.Agric.Univ.Perugia 38: 331-335, 1984. Billon-Grand, Mycotaxon 35: 201-204, 1989 (CoQ). Kurtzman, A.v.Leeuwenhoek 57: 215-222, 1990 (DNA). Rizzi et al., Appl.Microbiol.Biotechnol. 29: 148-154, 1988. Björling,T.,Lindman,B. Evaluation of xylose-fermenting yeasts for ethanol production from spent sulfite liquor.Enzyme and Microbial Technology. 11: 240-246, 1989 (Xylose). Dellweg,H et al. Xylose fermentation by yeasts 3. Comparison of Pachysolen tannophilus and Pichia stipitis.Biotechnology Letters. 6:395-400, 1984 (Xylose). Lee,H. et al. Utilization of xylan by yeasts and its conversion to ethanol by Pichia stipitis strain.Applied and Environmental Microbiology.52:320-324, 1986 (Xylan).
Information:	Production of ethanol from xylose. Convertion xylan into ethanol (Lee,H. et al. 1986)

Special Applications

Special Applications:	Degradation / Utilisation: Xylose Production: Ethanol

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Oval
Min Broth Breadth:	2
Max Broth Breadth:	4
Min Broth Length:	2
Max Broth Length:	5
Min Agar Breadth:	2
Max Agar Breadth:	5
Min Agar Length:	3
Max Agar Length:	5
Arrangement:	Single
Colour on Agar:	Cream
Surface on Agar:	Slightly shiny
Texture on Agar:	Smooth
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Incomplete
Ring Colour:	White
Pellicle in Broth:	Absent
Pellicle Appearance:
Pellicle Habitat:

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:
Blastospores:	Few
Blastospore Shape:	Oval
Blastospore Location:	Dispersed
Blastospore Habitat:	Single
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Absent
Ascospore Shape:
Ascospore Wall:
Ascospore No Per Ascus:
Ascus Shape:
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	No
Salt Tolerant:	-
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	Weak/Latent
Galactose:	Weak/Latent
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	Weak/Latent
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	Weak/Latent
Inulin:	-
Soluble Starch:	Weak/Latent
Xylose:	Unknown
A M D Glucoside:	Weak/Latent

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	+
Trehalose:	+
Lactose:	+
Melibiose:	-
Raffinose:	-
Melizitose:	+
Inulin:	-
Soluble Starch:	+
Xylose:	+
L Arabinose:	-
D Arabinose:	Weak/Latent
Ribose:	+
Rhamnose:	+
Ethanol:	+
Glycerol:	+
Erythritol:	+
Ribitol:	+
Galactitol:	-
Mannitol:	+
Sorbitol:	+
A M D Glucoside:	+
Salicin:	+
Lactic Acid:	-
Succinic Acid:	+
Citric Acid:	+
Inositol:	-
Gluconolactone:	+
Glucosamine:	Weak/Latent
Methanol:	-
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:

Vitamin Free Growth:	-
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	-
37c Growth:	-
40c Growth:
Arbutin Hydrolysis:	Weak/Latent
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	Unknown
Photograph:

Sequence Data:	Data not available.