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16 - Pichia subpelliculosa

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Strain Information

NCYC Number:	16
Name:	Pichia subpelliculosa
Depositor:	Dr. A. Harden
Deposit Name:	Hansenla subpelliculosa
Deposit Month:	June
Deposit Year:	1921
Equivalent Strain Designations:	ATCC 36905
Habitat:	Unknown
References:	Young TW, Yagiu. A comparison of the killer character in different yeasts and its classification. Antonie van Leeuwenhoek 44: 59-77, 1978. Chen WB , et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000.
Information:	Produces killer toxin (Young TW, Yagiu, 1978). sensitive to killer toxin produced by Schwanniomyces occidentalis ATCC 44252 (Chen WB, 2000).

Special Applications

Special Applications:	Production: Killer toxin

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Oval
Min Broth Breadth:	3
Max Broth Breadth:	5
Min Broth Length:	4
Max Broth Length:	14
Min Agar Breadth:	2
Max Agar Breadth:	5
Min Agar Length:	3
Max Agar Length:	12
Arrangement:	Single
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Rough
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thick
Pellicle Appearance:	Wrinkled
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:
Blastospores:	Absent
Blastospore Shape:
Blastospore Location:
Blastospore Habitat:
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	2
Ascus Shape:	Oval
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	No
Salt Tolerant:	15%
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	Weak/Latent
Sucrose:	+
Maltose:	Weak/Latent
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	Weak/Latent
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	Weak/Latent
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	+
Trehalose:	+
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	+
Inulin:	-
Soluble Starch:	+
Xylose:	+
L Arabinose:	-
D Arabinose:	-
Ribose:	+
Rhamnose:	-
Ethanol:	+
Glycerol:	+
Erythritol:	+
Ribitol:	+
Galactitol:	-
Mannitol:	+
Sorbitol:	+
A M D Glucoside:	+
Salicin:	+
Lactic Acid:	+
Succinic Acid:	+
Citric Acid:	+
Inositol:	-
Gluconolactone:	+
Glucosamine:	-
Methanol:	-
Xylitol:	+

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	+
Ethylamine:	+
Cadaverine:	+
Lysine:

Vitamin Free Growth:	+
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	Weak/Latent
37c Growth:	-
40c Growth:
Arbutin Hydrolysis:	+
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Sequence Data:	Data not available.