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169 - Pichia membranifaciens

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Strain Information

NCYC Number:	169
Name:	Pichia membranifaciens
Depositor:	T. Castelli
Deposit Name:	Zygopichia chiantigiana
Deposit Month:	March
Deposit Year:	1939
Equivalent Strain Designations:	NCTC 5827
Habitat:	Chianti grape must.

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Elongated
Min Broth Breadth:	4
Max Broth Breadth:	5
Min Broth Length:	5
Max Broth Length:	10
Min Agar Breadth:	3
Max Agar Breadth:	6
Min Agar Length:	10
Max Agar Length:	13
Arrangement:	Pairs
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Smooth
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Not Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:	Unknown
Blastospores:	Absent
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habitat:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Round
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	1
Ascus Shape:	Round
Conjugation:	Present
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	No
Salt Tolerant:	-
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	-
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	+
Sorbitol:	+
A M D Glucoside:	-
Salicin:	-
Lactic Acid:	-
Succinic Acid:	Weak/Latent
Citric Acid:	-
Inositol:	-
Gluconolactone:	-
Glucosamine:	-
Methanol:	-
Xylitol:	-

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:	Unknown

Vitamin Free Growth:	Weak/Latent
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	Weak/Latent
37c Growth:	-
40c Growth:	Unknown
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	Weak/Latent

Sequence Data:	Data not available.