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235 - Saccharomyces cerevisiae

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Strain Information

This is a brewing production strain

NCYC Number:	235
Name:	Saccharomyces cerevisiae
Depositor:	B.M. Brown
Deposit Name:	Saccharomyces cerevisiae
Deposit Month:	May
Deposit Year:	1953
Equivalent Strain Designations:	Whitbread Yeast Z S.C. 169, ATCC 60733
Habitat:	Ale production yeast.
References:	Young TW , Yagiu M . A comparison of the killer character in different yeasts and its classification. Antonie van Leeuwenhoek 44: 59-77, 1978. Chen WB , et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000.
Information:	Produces group K1 killer toxin. Sensitive to killer toxin produced by Schwanniomyces occidentalis ATCC 44252.

Special Applications

Special Applications:	Brewing: Ale Production: Killer toxin

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Round-oval
Min Broth Breadth:	5
Max Broth Breadth:	8
Min Broth Length:	5
Max Broth Length:	10
Min Agar Breadth:	4
Max Agar Breadth:	5
Min Agar Length:	5
Max Agar Length:	9
Arrangement:	Chains
Colour on Agar:	Cream
Surface on Agar:	Shiny
Texture on Agar:	Smooth
Deposit in Broth:	Unknown
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Absent
Pellicle Appearance:	N/A
Pellicle Habitat:	N/A

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Absent
Pseudomycelium Branch:	N/A
Pseudomycelium Form:	N/A
Blastospores:	Absent
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habitat:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Round
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	2
Ascus Shape:	Oval
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	Unknown
Salt Tolerant:	Unknown
Killer:	Yes
Plasmid:	No

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	+
Sucrose:	+
Maltose:	+
Cellobiose:	Unknown
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	+
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	Unknown

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	Unknown
Sucrose:	+
Maltose:	+
Cellobiose:	-
Trehalose:	+
Lactose:	-
Melibiose:	-
Raffinose:	Unknown
Melizitose:	-
Inulin:	-
Soluble Starch:	Unknown
Xylose:	-
L Arabinose:	Unknown
D Arabinose:	-
Ribose:	-
Rhamnose:	Unknown
Ethanol:	+
Glycerol:	-
Erythritol:	Unknown
Ribitol:	Unknown
Galactitol:	Unknown
Mannitol:	-
Sorbitol:	Unknown
A M D Glucoside:	+
Salicin:	-
Lactic Acid:	+
Succinic Acid:	-
Citric Acid:	Unknown
Inositol:	Unknown
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	-
Ethylamine:	-
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	Unknown
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	Unknown
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	Unknown
37c Growth:	Unknown
40c Growth:	Unknown
Arbutin Hydrolysis:	-
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown

Brewing Strain Information

Head:	good head
Deposit:	< 8mm
Attenuation:	1.008-1.010
Fermentation Rate:	6-8°
Clarity:	>20
Flocculence:
Date of Deposit:
Description:

Sequence Data:	Data not available.