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3309 - Issatchenkia orientalis

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Strain Information

NCYC Number:	3309
Name:	Issatchenkia orientalis
Depositor:	Dr. M. Stratford, Unilever Research, UK.
Deposit Name:	Issatchenkia orientalis
Deposit Month:	May
Deposit Year:	2005
Equivalent Strain Designations:	Uni 522
Habitat:	Drain in a Brazilan soft drinks factory.^

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Oval to Elongated
Min Broth Breadth:	4
Max Broth Breadth:	6
Min Broth Length:	5
Max Broth Length:	16
Min Agar Breadth:	4
Max Agar Breadth:	6
Min Agar Length:	5
Max Agar Length:	18
Arrangement:	Singly, in Pairs & Chains .
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Slightly Rough
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Absent
Ring Colour:	N/A
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Partially Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Irregularly branched
Pseudomycelium Form:	Candida + Mycotoruloides
Blastospores:	Many
Blastospore Shape:	Oval
Blastospore Location:	Unknown
Blastospore Habitat:	Clusters
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Unknown
Ascospore Shape:	Unknown
Ascospore Wall:	Unknown
Ascospore No Per Ascus:	Unknown
Ascus Shape:	Unknown
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	Unknown
Salt Tolerant:	<10%
Killer:	Unknown
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	Unknown
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	+
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	-
Salicin:	-
Lactic Acid:	+
Succinic Acid:	+
Citric Acid:	-
Inositol:	-
Gluconolactone:	+
Glucosamine:	-
Methanol:	-
Xylitol:	-

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:	+

Vitamin Free Growth:	Unknown
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	+
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	-
37c Growth:	+
40c Growth:	+
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Sequence Data:	Data not available.