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333 - Pichia membranifaciens

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Strain Information

This strain is recommended for studies involving Pichia membranifaciens

NCYC Number:	333
Name:	Pichia membranifaciens
Depositor:	A.E. Wiles
Deposit Name:	Pichia alcoholophila
Deposit Month:	October
Deposit Year:	1951
Equivalent Strain Designations:	ATCC 36908, CBS 7374, 'Strain 79'
Habitat:	Draught beer, UK.
References:	Young, in Rose & Harrison, The Yeasts Vol.2, London: Academic Press. Wiles, J. Inst. Brew. 56: 183, 1950. Killer character K7, A.v. Leeuwenhoek, 1978, 44, 59-77. Killer properties of wine yeasts. Shimizu et al. Journal of Fermentation Technology, 63, 421-429, 1985.
Information:	Killer character K7 (A.v. Leeuwenhoek, 1978, 44, 59-77; Shimizu et al. 1985).

Special Applications

Special Applications:	Production: Killer toxin

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Long-oval
Min Broth Breadth:	3
Max Broth Breadth:	5
Min Broth Length:	5
Max Broth Length:	8
Min Agar Breadth:	2
Max Agar Breadth:	5
Min Agar Length:	4
Max Agar Length:	12
Arrangement:	Pairs
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Rough
Deposit in Broth:	Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Unknown
Pseudomycelium Form:	Unknown
Blastospores:	Unknown
Blastospore Shape:	Oval
Blastospore Location:	Unknown
Blastospore Habitat:	Unknown
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	4
Ascus Shape:	Unknown
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	Unknown
Salt Tolerant:	10%
Killer:	Yes
Plasmid:	Yes

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	Unknown
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	Unknown
Xylose:	Weak/Latent
L Arabinose:	Unknown
D Arabinose:	Unknown
Ribose:	Unknown
Rhamnose:	-
Ethanol:	Unknown
Glycerol:	Unknown
Erythritol:	-
Ribitol:	-
Galactitol:	Unknown
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	Unknown
Salicin:	Unknown
Lactic Acid:	Unknown
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	Unknown
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	-
Ethylamine:	Unknown
Cadaverine:	Unknown
Lysine:

Vitamin Free Growth:	+
Cyclohex 100ppm:	Unknown
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	Unknown
Lipolytic:	-
Acid Production:	Unknown
37c Growth:	Unknown
40c Growth:
Arbutin Hydrolysis:	-
Urease Activity:	Unknown
Starch Production:	Unknown
Acid Tolerant:	Weak/Latent

Sequence Data:	Data not available.