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334 - Pichia membranifaciens

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Strain Information

NCYC Number:	334
Name:	Pichia membranifaciens
Depositor:	A.E. Wiles
Deposit Name:	Pichia belgica
Deposit Month:	October
Deposit Year:	1951
Equivalent Strain Designations:	T80
Habitat:	Draught beer, UK.
References:	Wiles, J. Inst. Brew. 56: 183, 1950.

Special Applications

Special Applications:	Tolerance: Benzoic acid (300ppm)

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Long-oval
Min Broth Breadth:	3
Max Broth Breadth:	5
Min Broth Length:	3
Max Broth Length:	9
Min Agar Breadth:	5
Max Agar Breadth:	5
Min Agar Length:	6
Max Agar Length:	11
Arrangement:	Pairs
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Wrinkled
Deposit in Broth:	Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thick
Pellicle Appearance:	Wrinkled
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Unknown
Pseudomycelium Form:	Unknown
Blastospores:	Few
Blastospore Shape:	Unknown
Blastospore Location:	Unknown
Blastospore Habitat:	Unknown
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hemispherical
Ascospore Wall:	Unknown
Ascospore No Per Ascus:	4
Ascus Shape:	Unknown
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	Unknown
Salt Tolerant:	Unknown
Killer:	Unknown
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	Unknown
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	Unknown
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	Unknown
Xylose:	Weak/Latent
L Arabinose:	-
D Arabinose:	Unknown
Ribose:	Unknown
Rhamnose:	-
Ethanol:	Unknown
Glycerol:	Unknown
Erythritol:	-
Ribitol:	-
Galactitol:	Unknown
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	Unknown
Salicin:	Unknown
Lactic Acid:	Unknown
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	Unknown
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	-
Ethylamine:	Unknown
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	-
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	-
37c Growth:	-
40c Growth:	-
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Sequence Data:	Data not available.