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432 - Pichia anomala

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Strain Information

This is the Pichia anomala Type Strain

This strain is recommended for studies involving Pichia anomala

NCYC Number:	432
Name:	Pichia anomala
Depositor:	L.J. Wickerham, NRRL, USA.
Deposit Name:	Hansenula anomala
Deposit Month:	March
Deposit Year:	1955
Equivalent Strain Designations:	ATCC 8168, CBS 5759, CCRC 22583, DBVPG 6781, IFO 10213, IGC 4121, JCM 3585, NRRL Y-366.
Habitat:	Not specified
References:	Perez JA, et al. Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of hypoglycosylated invertase. Arch. Microbiol. 175: 189-197, 2001. Aono R. Taxonomic distribution of alkali-tolerant yeasts. Syst. Appl. Microbiol. 13: 394-397, 1990. Bellinger Y, Larher F. Factors influencing the adaptation of the yeast Hansenula anomala var. anomala (CBS 5759) to galactose assimilation. C.R. Acad. Sci. Paris, Ser III 310: 551-555, 1990.
Information:	Produces beta-fructofuranosidase (beta-fructosidase). Expression of INV1 gene in Saccharomyces cerevisiae (Perez JA, et al., 2001). Alkali-tolerant at pH 10 (Aono R, 1990). Galactose assimilation (Bellinger Y, Larher F, 1990). K4 type killer.

Special Applications

Special Applications:	Production: Beta-fructofuranosidase Production: Killer toxin

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Round-oval
Min Broth Breadth:	3
Max Broth Breadth:	5
Min Broth Length:	4
Max Broth Length:	6
Min Agar Breadth:	4
Max Agar Breadth:	5
Min Agar Length:	3
Max Agar Length:	5
Arrangement:	Single
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Wrinkled
Deposit in Broth:	Unknown
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:
Pseudomycelium Form:	Mycotorula
Blastospores:	Unknown
Blastospore Shape:	Round
Blastospore Location:	Unknown
Blastospore Habitat:	Unknown
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	4
Ascus Shape:	Unknown
Conjugation:	Absent
Teliospores:	Unknown
Teliospore Shape:	Unknown

Assay:	Unknown
Salt Tolerant:	Unknown
Killer:	K4
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	Weak/Latent
Sucrose:	+
Maltose:	+
Cellobiose:	Unknown
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	+
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	Unknown
Xylose:	Unknown
A M D Glucoside:	Unknown

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	Unknown
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	Unknown
Melizitose:	Unknown
Inulin:	-
Soluble Starch:	+
Xylose:	Unknown
L Arabinose:	-
D Arabinose:	-
Ribose:	Unknown
Rhamnose:	-
Ethanol:	Unknown
Glycerol:	Unknown
Erythritol:	+
Ribitol:	Weak/Latent
Galactitol:	Unknown
Mannitol:	Unknown
Sorbitol:	Unknown
A M D Glucoside:	Unknown
Salicin:	Unknown
Lactic Acid:	Unknown
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	-
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	+
Ethylamine:	Unknown
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	+
Cyclohex 100ppm:	Unknown
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	Unknown
Lipolytic:
Acid Production:	Unknown
37c Growth:	Unknown
40c Growth:	Unknown
Arbutin Hydrolysis:	+
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown
Photograph:

Sequence Data:	Data not available.