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44 - Pichia membranifaciens

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Strain Information

NCYC Number:	44
Name:	Pichia membranifaciens
Depositor:	A. Harden, Lister Institute.
Deposit Name:	Mycoderma cerevisiae
Deposit Month:	January
Deposit Year:	1921
Equivalent Strain Designations:	NCTC 819
Habitat:	Unknown
References:	A.E. Wiles, J.Inst. Brew., 1950, 56, p.192. J. Inst. Brew., 1952, 58, p.140.

Special Applications

Special Applications:	Tolerance: Benzoic acid (300ppm)

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):	27.5
Miniumum Temperature(Degrees Celcius):	11
Maximum Temperature (Degrees Celcius):	32.2

Cells:

Shape:	Oval
Min Broth Breadth:	2
Max Broth Breadth:	4
Min Broth Length:	3
Max Broth Length:	8
Min Agar Breadth:	3
Max Agar Breadth:	5
Min Agar Length:	4
Max Agar Length:	6
Arrangement:	Single
Colour on Agar:	Cream
Surface on Agar:	Dull
Texture on Agar:	Rough
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thick
Pellicle Appearance:	Wrinkled
Pellicle Habitat:	Not Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Regularly branched
Pseudomycelium Form:
Blastospores:	Few
Blastospore Shape:	Oval
Blastospore Location:	Dispersed
Blastospore Habitat:	Single
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:	Absent
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	4
Ascus Shape:	Round
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	No
Salt Tolerant:	10%
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	Weak/Latent
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	-
Salicin:	-
Lactic Acid:	Weak/Latent
Succinic Acid:	-
Citric Acid:	-
Inositol:	-
Gluconolactone:	-
Glucosamine:	-
Methanol:	-
Xylitol:	-

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:

Vitamin Free Growth:	-
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:	-
Acid Production:	-
37c Growth:	-
40c Growth:
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Sequence Data:	Data not available.