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497 - Pichia canadensis

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Strain Information

This is the Pichia canadensis Type Strain

This strain is recommended for studies involving Pichia canadensis

NCYC Number:	497
Name:	Pichia canadensis
Depositor:	L.J. Wickerham, NRRL.
Deposit Name:	Hansenula canadensis
Deposit Month:	April
Deposit Year:	1957
Equivalent Strain Designations:	ATCC 18079, CBS 1992, CCRC 20482, CCRC 22407, DBVPG 6262, IFO 0973, JCM 3597, NRRL Y-1888, UCD 74-71
Habitat:	Insect frass on Pinus resinosa (red pine), Ontario, Canada.
References:	Wickerham, US.Dep.Agric.Tech.Bull.1029, Washington 1951, p.26. Kurtzman, Mycologia 79: 410-417, 1987 (DNA). Fuson et al., Int.J.Syst.Bacteriol. 29: 64-69, 1979 (DNA). Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ. Hou CT. pH dependence and thermostability of lipases from cultures from the ARS Culture Collection. J. Ind. Microbiol. 13: 242-248, 1994.
Information:	Produces lipases active at pH 9.0 at 60C (Hou CT, 1994).

Special Applications

Special Applications:	Production: Lipase

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Oval
Min Broth Breadth:	2
Max Broth Breadth:	3
Min Broth Length:	2
Max Broth Length:	5
Min Agar Breadth:	1
Max Agar Breadth:	3
Min Agar Length:	2
Max Agar Length:	5
Arrangement:	Pairs
Colour on Agar:	White
Surface on Agar:	Shiny
Texture on Agar:	Smooth
Deposit in Broth:	Non-Flocculent
Ring in Broth:	Complete
Ring Colour:	White
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Unknown
Pseudomycelium Form:	Mycotorula
Blastospores:	Many
Blastospore Shape:	Oval
Blastospore Location:	Verticils
Blastospore Habitat:	Unknown
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	2
Ascus Shape:	Unknown
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	Unknown

Assay:	Unknown
Salt Tolerant:	-
Killer:	Unknown
Plasmid:	No

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	Weak/Latent
Sucrose:	+
Maltose:	+
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	+
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	+
Maltose:	+
Cellobiose:	Unknown
Trehalose:	+
Lactose:	-
Melibiose:	Unknown
Raffinose:	+
Melizitose:	+
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
L Arabinose:	-
D Arabinose:	-
Ribose:	Unknown
Rhamnose:	+
Ethanol:	Unknown
Glycerol:	Unknown
Erythritol:	-
Ribitol:	-
Galactitol:	Unknown
Mannitol:	Unknown
Sorbitol:	Unknown
A M D Glucoside:	+
Salicin:	Unknown
Lactic Acid:	Unknown
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	Unknown
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	+
Ethylamine:	Unknown
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	-
Cyclohex 100ppm:	Unknown
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	Unknown
Lipolytic:
Acid Production:	Unknown
37c Growth:	Unknown
40c Growth:	Unknown
Arbutin Hydrolysis:	Weak/Latent
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown
Photograph:

Sequence Data:	Data not available.