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546 - Kluyveromyces wickerhamii

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Strain Information

This is the Kluyveromyces wickerhamii Type Strain

This strain is recommended for studies involving Kluyveromyces wickerhamii

NCYC Number:	546
Name:	Kluyveromyces wickerhamii
Depositor:	Dr. H. J. Phaff
Deposit Name:	Saccharomyces wickerhamii
Deposit Month:	November
Deposit Year:	1958
Equivalent Strain Designations:	Phaff strain F-394A - Believed to be the same srtain as: ATCC 24178, CBS 2745, DBVPG 6077, IFO 1675, NRRL Y-8286, UCD 54-210.
Habitat:	Drosophila sp. (fruit fly), California, USA.
References:	Yoshitake S et al. Purification and characterization of a pectin-releasing enzyme produced by Kluyveromyces wickerhamii. J. Ferment. Bioeng. 77: 370-375, 1994. Phaff HJ et al. The taxonomy of yeasts isolated from Drosophila in the Yosemite region of California. Antonie van Leeuwenhoek 22: 145-161, 1956.
Information:	Type strain of Saccharomyces wickerhamii. Produces pectin-releasing enzyme PPase-W (Yoshitake S, 1994).

Special Applications

Special Applications:	Production: Pectin-releasing enzyme

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Long-oval
Min Broth Breadth:	2
Max Broth Breadth:	4
Min Broth Length:	4
Max Broth Length:	6
Min Agar Breadth:	2
Max Agar Breadth:	5
Min Agar Length:	3
Max Agar Length:	8
Arrangement:	Pairs
Colour on Agar:	Cream
Surface on Agar:	Shiny
Texture on Agar:	Smooth
Deposit in Broth:	Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Unknown

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Absent
Pseudomycelium Branch:	N/A
Pseudomycelium Form:	N/A
Blastospores:	N/A
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habitat:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Reniform
Ascospore Wall:	Unknown
Ascospore No Per Ascus:	4
Ascus Shape:	Unknown
Conjugation:	Present
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	Unknown
Salt Tolerant:	Unknown
Killer:	Unknown
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	+
Galactose:	+
Sucrose:	+
Maltose:	-
Cellobiose:	Unknown
Trehalose:	Unknown
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	Unknown

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	Unknown
Sucrose:	+
Maltose:	Unknown
Cellobiose:	Weak/Latent
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	Unknown
Inulin:	Unknown
Soluble Starch:	Unknown
Xylose:	+
L Arabinose:	Unknown
D Arabinose:	Unknown
Ribose:	Unknown
Rhamnose:	Unknown
Ethanol:	Unknown
Glycerol:	Unknown
Erythritol:	Unknown
Ribitol:	Unknown
Galactitol:	Unknown
Mannitol:	-
Sorbitol:	Weak/Latent
A M D Glucoside:	Unknown
Salicin:	+
Lactic Acid:	-
Succinic Acid:	Unknown
Citric Acid:	Unknown
Inositol:	Unknown
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	Unknown
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	Unknown
KNO3:	-
Ethylamine:	+
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	Weak/Latent
Cyclohex 100ppm:	+
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	-
Lipolytic:
Acid Production:	Unknown
37c Growth:	Unknown
40c Growth:	Unknown
Arbutin Hydrolysis:	Weak/Latent
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown
Photograph:

Sequence Data:	Data not available.