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55 - Pichia membranifaciens

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Strain Information

NCYC Number:	55
Name:	Pichia membranifaciens
Depositor:	A. Klocker, Carlsberg laboratories, Copenhagen.
Deposit Name:	Pichia membranaefaciens var. calliphorae
Deposit Month:	April
Deposit Year:	1920
Equivalent Strain Designations:	NCTC 483
Habitat:	Fly (Calliphora erythrocephala) found in garden near Carlsberg. Isolated 1900.
References:	C.R. Lab. Carlsberg, 1911-17, 10, 211. A.E.Wiles J.I.B. 1950. p192.

Special Applications

Special Applications:	Tolerance: Benzoic acid (300ppm)

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Long-oval
Min Broth Breadth:	2
Max Broth Breadth:	4
Min Broth Length:	5
Max Broth Length:	14
Min Agar Breadth:	3
Max Agar Breadth:	4
Min Agar Length:	9
Max Agar Length:	13
Arrangement:	Pairs
Colour on Agar:	Yellow
Surface on Agar:	Dull
Texture on Agar:	Wrinkled
Deposit in Broth:	Flocculent
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Well formed
Pseudomycelium Branch:	Regularly branched
Pseudomycelium Form:
Blastospores:	Absent
Blastospore Shape:
Blastospore Location:
Blastospore Habitat:
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Present
Ascospore Shape:	Hat
Ascospore Wall:	Smooth
Ascospore No Per Ascus:	2
Ascus Shape:	Oval
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:

Assay:	No
Salt Tolerant:	10%
Killer:	No
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	-
Sorbose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	-
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	+
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	-
Sorbitol:	-
A M D Glucoside:	-
Salicin:	-
Lactic Acid:	Weak/Latent
Succinic Acid:	+
Citric Acid:	-
Inositol:	-
Gluconolactone:	-
Glucosamine:	-
Methanol:	-
Xylitol:	-

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	+
Lysine:

Vitamin Free Growth:	+
Cyclohex 100ppm:	-
Cyclohex 1000ppm:	-
50% Glucose Growth:	-
60% Glucose Growth:	-
Lipolytic:
Acid Production:	-
37c Growth:	-
40c Growth:
Arbutin Hydrolysis:	-
Urease Activity:	-
Starch Production:	-
Acid Tolerant:	-

Sequence Data:	Data not available.