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770 - Trigonopsis variablis

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Strain Information

This is the Trigonopsis variablis Type Strain

This strain is recommended for studies involving Trigonopsis variablis

NCYC Number:	770
Name:	Trigonopsis variablis
Depositor:	J.A. Barnett
Deposit Name:	Trigonopsis variablis
Deposit Month:	January
Deposit Year:	1974
Equivalent Strain Designations:	ATCC 10679, CBS 1040, CCRC 21509, DSM 70714, DBVPG 6199, IFO 0755, JCM 1823, NRRL Y-1579, VKM Y-825, VKM Y-2601
Habitat:	Isolated from beer, Germany.
References:	Packer & Bersten, J.Gen.Microbiol. 101: 233-236, 1977. FEMS Microbiol.Lett. 12: 135-138, 1981 (Morphogenesis) Nakase & Komagata, J.Gen.Appl.Microbiol. 17: 363-369, 1971 (GC). Yamada et al., J.Gen.Appl.Microbiol. 29: 51-57, 1983 (CoQ). Berg CP , Rodden FA . Purification of D-amino acid oxidase from Trigonopsis variabilis. Anal. Biochem. 71: 214-222, 1976. Patel RN , et al. Microbial reduction of 1-(4-fluorophenyl)-4-[4-(5-fluoro-2-pyrimidinyl)-1-piperazinyl]butan-1-one. Biotechnol. Appl. Biochem. 17: 139-153, 1993. Schachner J . Trigonopsis variabilis nov. gen. et spec.. Z. Gesamte Brauwesen 52: 137-142, 1929. Wong BL , Shen YQ . Production of 7-amino cephalosporanic acid with D-amino oxidase and deacylase. US Patent 5,618,687 dated Apr 8 1997
Information:	Produces D-amino acid oxidase ( Berg CP , 1976; Wong BL , Shen YQ, 1997.) Reduces 4-chloro-1-(4-fluorophenyl) butan-1-one (Patel RN , et al, 1993).

Special Applications

Special Applications:	Production: D-amino acid oxidase

Physical Characteristics

Temperature:

Optimum Temperature (Degrees Celcius):
Miniumum Temperature(Degrees Celcius):
Maximum Temperature (Degrees Celcius):

Cells:

Shape:	Triangular
Min Broth Breadth:	3
Max Broth Breadth:	8
Min Broth Length:	3
Max Broth Length:	8
Min Agar Breadth:	4
Max Agar Breadth:	7
Min Agar Length:	4
Max Agar Length:	7
Arrangement:	Pairs
Colour on Agar:	White
Surface on Agar:	Dull
Texture on Agar:	Rough
Deposit in Broth:	Unknown
Ring in Broth:	Absent
Ring Colour:	Unknown
Pellicle in Broth:	Thin
Pellicle Appearance:	Milky
Pellicle Habitat:	Climbing

Cell Division:

Budding:	Multipolar
Fission:	Absent

Filamentous Growth:

Pseudomycelium:	Absent
Pseudomycelium Branch:	N/A
Pseudomycelium Form:	N/A
Blastospores:	N/A
Blastospore Shape:	N/A
Blastospore Location:	N/A
Blastospore Habitat:	N/A
True Mycelium:	Absent
Clamp Connections:	Absent

Asexual Spores:

Ballistospores:	Absent
Arthrospores:
Endospores:	Absent
Chlamydospores:	Absent

Sexual Spores:

Ascospores:	Absent
Ascospore Shape:	N/A
Ascospore Wall:	N/A
Ascospore No Per Ascus:	N/A
Ascus Shape:	N/A
Conjugation:	Absent
Teliospores:	Absent
Teliospore Shape:	N/A

Assay:	Unknown
Salt Tolerant:	Unknown
Killer:	Unknown
Plasmid:	Unknown

Semi-Anaerobic Fermentation

Glucose:	-
Galactose:	-
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	-
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	Unknown
Soluble Starch:	-
Xylose:	Unknown
A M D Glucoside:	-

Aerobic Utilisation and Growth

Glucose:	+
Galactose:	+
Sorbose:	+
Sucrose:	-
Maltose:	-
Cellobiose:	-
Trehalose:	+
Lactose:	-
Melibiose:	-
Raffinose:	-
Melizitose:	-
Inulin:	-
Soluble Starch:	-
Xylose:	-
L Arabinose:	+
D Arabinose:	-
Ribose:	-
Rhamnose:	-
Ethanol:	+
Glycerol:	+
Erythritol:	-
Ribitol:	-
Galactitol:	-
Mannitol:	+
Sorbitol:	+
A M D Glucoside:	-
Salicin:	-
Lactic Acid:	Weak/Latent
Succinic Acid:	+
Citric Acid:	Unknown
Inositol:	-
Gluconolactone:	Unknown
Glucosamine:	Unknown
Methanol:	-
Xylitol:	Unknown

Sole Sources of Nitrogen

NH4 2SO4:	+
KNO3:	-
Ethylamine:	+
Cadaverine:	Unknown
Lysine:	Unknown

Vitamin Free Growth:	-
Cyclohex 100ppm:	+
Cyclohex 1000ppm:	Unknown
50% Glucose Growth:	Unknown
60% Glucose Growth:	-
Lipolytic:
Acid Production:	Weak/Latent
37c Growth:	+
40c Growth:	Unknown
Arbutin Hydrolysis:	-
Urease Activity:	Unknown
Starch Production:	-
Acid Tolerant:	Unknown
Photograph:

Sequence Data:	Data not available.