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NCYC 175

Komagataella pastoris

Pre 2011 Name

Pichia pastoris

Equivalent Strain Designations

ATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.

Depositor

A. Guilliermond

Deposit Date

Unknown 1925

Habitat

Exudate of Chestnut tree, near Lyon, France.
Komagataella pastoris

Details

Komagataella pastoris

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Key Information

Aerobic Utilisation and Growth

  • Glucose +
  • Galactose -
  • Sorbose -
  • Sucrose -
  • Maltose -
  • Cellobiose -
  • Trehalose +
  • Lactose -
  • Melibiose -
  • Raffinose -
  • Melezitose -
  • Inulun -
  • Soluble Starch -
  • Xylose -
  • L Arabinose -
  • D Arabinose -
  • Ribose -
  • Rhamnose +
  • Ethanol +
  • Glycerol +
  • Erythritol -
  • Ribitol -
  • Galactitol -
  • Mannitol +
  • SorbitolWeak/Latent
  • AMD Glucoside -
  • Salicin -
  • Lactic AcidWeak/Latent
  • Succinic Acid +
  • Citric Acid +
  • Inositol -
  • Gluconolactone -
  • Glucosamine -
  • Methanol +
  • Xylitol -

Strain Information

  • Information

    Production of yeast protein from hydrocarbons (US Patent 4,226,939). Produces natural flavor aldehydes (Murray WD, 1989). Converts ethanol to acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Degrades ethyl alcohol (ethanol) (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Degrades hydrogen peroxide (Duff SJB, Murray WD, 1990). Produces acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Produces alcohol oxidase (Murray WD, et al, 1989; Murray WD, et al, 1990; Agric. Biol. Chem. 54: 1879-1882, 1990).

  • DepositorA. Guilliermond
  • Deposit NameSaccharomyces pastori / Zygosaccharomyces pastori
  • Month of depositUnknown
  • Deposit Year1925
  • HabitatExudate of Chestnut tree, near Lyon, France.
  • Equivalent Strain DesignationsATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.
  • ReferenceGuilliermond, CR.Hebd. Séances Mem.Soc.Biol. 82: 466-470, 1919. Nakase & Komagata, J.Gen.Appl.Microbiol. 16: 511-521, 1970 (GC). Bicknell & Douglas, J.Bacteriol. 101: 505, 1970 (GC). Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ). Duff SJ, Murray WD. Production and application of methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 31: 44-49, 1988a. Duff SJ, Murray WD. Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde. Biotechnol. Bioeng. 31: 790-795, 1988b. Duff SJ, Murray WD. Production of flavor aldehydes using nongrowing whole cells of Pichia pastoris. Ann. N.Y. Acad. Sci. 542: 428-433, 1988c. Murray WD, et al. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32: 95-100, 1989. Duff SJ, Murray WD. Oxidation of benzyl alcohol by whole cells of Pichia pastoris and by alcohol oxidase in aqueous and nonaqueous reaction media. Biotechnol. Bioeng. 34: 153-159, 1989. Murray WD, et al. Catabolite inactivation in the methylotrophic yeast Pichia pastoris. Appl. Environ. Microbiol. 56: 2378-2383, 1990. Wegner EH. Treatment of make-up water for use in a fermentation process for growth of yeast cells requiring growth factors. US Patent 4,226,939 dated Oct 7 1980 Murray WD, et al. Production of natural flavor aldehydes from natural source primary alcohols C2-C7. US Patent 4,871,669 dated Oct 3 1989 Phaff HJ. A proposal for amendment of the diagnosis of the genus Pichia Hansen. Antonie van Leeuwenhoek 22: 113-116, 1956. Appl. Microbiol. Biotechnol. 36: 82-86, 1991. Yamada Y, et al. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci. Biotechnol. Biochem. 59: 439-444, 1995. Enzyme Microb. Technol. 11: 770-775, 1989. Agric. Biol. Chem. 54: 1879-1882, 1990. Duff SJB, Murray WD. Metabolism of hydrogen peroxide by Pichia pastoris. Agric. Biol. Chem. 54: 1967-1974, 1990.

Physical Characteristics

  • Optimum TemperatureUnknown
  • Miniumum TemperatureUnknown
  • Maximum TemperatureUnknown

Cells

  • ShapeOval
  • Min Broth Breadth (µm)3
  • Max Broth Breadth (µm)5
  • Min Broth Length (µm)3
  • Max Broth Length (µm)6
  • Min Agar Breadth (µm)2
  • Max Agar Breadth (µm)5
  • Min Agar Length (µm)3
  • Max Agar Length (µm)6
  • ArrangementPairs
  • Colour on AgarCream
  • Surface on AgarShiny
  • Texture on AgarSmooth
  • Deposit in BrothNon-Flocculent
  • Ring in BrothIncomplete
  • Ring ColourWhite
  • Pellicle in BrothAbsent
  • Pellicle AppearanceN/A
  • Pellicle HabitatN/A

Cell Division

  • BuddingMultipolar
  • FissionAbsent

Filamentous Growth

  • PseudomyceliumWell formed
  • Pseudomycelium BranchIrregularly branched
  • Pseudomycelium FormMycocandida
  • BlastosporesAbsent
  • Blastospore ShapeN/A
  • Blastospore LocationN/A
  • Blastospore HabitatN/A
  • True MyceliumAbsent
  • Clamp ConnectionsAbsent

Asexual Spores

  • BallistosporesAbsent
  • AthrosporesAbsent
  • EndosporesAbsent
  • ChlamydosporesAbsent

Sexual Spores

  • AscosporesPresent
  • Ascospore ShapeHat
  • Ascospore WallUnknown
  • Ascospores No Per Ascus4
  • Ascus ShapeUnknown
  • ConjugationPresent
  • TeliosporesAbsent
  • Teliospore ShapeN/A

Miscellaneous

  • AssayUnknown
  • Salt TolerantUnknown
  • KillerNo
  • PlasmidNo

Semi-Anaerobic Fermentation

  • Glucose +
  • Galactose -
  • Sucrose -
  • Maltose -
  • Cellobiose -
  • Trehalose -
  • Lactose -
  • Melibiose -
  • Raffinose -
  • Melizitose -
  • Inulin -
  • Soluble Starch -
  • XyloseUnknown
  • AMD Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

  • NH4 2SO4 +
  • KNO3 -
  • Ethylamine +
  • Cadaverine +
  • LysineUnknown

Other

  • Vitamin Free GrowthWeak/Latent
  • Cyclohex 100 +
  • Cyclohex 1000 +
  • Glucose Growth 50 -
  • Glucose Growth 60 -
  • Lipolytic -
  • Acid ProductionWeak/Latent
  • Growth 37 -
  • Growth 40Unknown
  • Arbutin Hydrolysis -
  • Urease Activity -
  • Starch Production -
  • Acid TolerantUnknown

Genomic Sequence Data

No data