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NCYC 416

Kluyveromyces lactis var. lactis

Taxon Synonyms

Kluyveromyces lactis, Torulaspora lactis, Zygosaccharomyces lactis

Equivalent Strain Designations

ATCC 56498, CBS 683, DBVPG 6305, DSM 4394, IFO 1090, NRRL Y-8279, UCD 71-59.

Depositor

CBS

Deposit Date

1955

Habitat

Gassy cheese. (UK)

Details

Kluyveromyces lactis var. lactis

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Key Information

Aerobic Utilisation and Growth

  • Glucose+
  • Galactose+
  • Sorbose-
  • Sucrose+
  • Maltose+
  • Cellobiose+
  • Trehalose+
  • Lactose+
  • Melibiose-
  • Raffinose+
  • Melezitose+
  • Inulun-
  • Soluble Starch-
  • XyloseWeak/Latent
  • L Arabinose-
  • D Arabinose-
  • Ribose-
  • Rhamnose-
  • Ethanol+
  • Glycerol+
  • Erythritol-
  • Ribitol-
  • Galactitol-
  • Mannitol+
  • Sorbitol+
  • AMD Glucoside+
  • Salicin+
  • Lactic Acid+
  • Succinic Acid+
  • Citric Acid-
  • Inositol-
  • Gluconolactone-
  • GlucosamineWeak/Latent
  • Methanol-
  • Xylitol+

Strain Information

  • Information

    MT alpha. Campbell serotype C. Produces beta-galactosidase (beta-D-galactosidase) (Grieve PA, et al., 1983; Inchaurrondo VA, 1994). Production of cheese-ripening proteinases (Grieve PA, et al., 1983). Transformation host (Blondeau K, 1994; Appl. Microbiol. Biotechnol. 40: 306-308, 1993; Biotechnol. Tech. 7: 609-614, 1993). Disinfectant testing.

  • DepositorCBS
  • Deposit NameSaccharomyces lactis
  • Month of depositUnknown
  • Deposit Year1955
  • HabitatGassy cheese. (UK)
  • Equivalent Strain DesignationsATCC 56498, CBS 683, DBVPG 6305, DSM 4394, IFO 1090, NRRL Y-8279, UCD 71-59.
  • ReferenceAllen & Thornley Ann.Appl.Biol. 16: 578, 1929. Steensma, Curr.Genet. 14: 311-317. GenLit: Coria et al, A.v.Leeuwenhoek 58: 227-234, 1990 (mtDNA). Cong et al., Microbiology 140: 1327-1335, 1994 (plasmid). Vaughan-Martini, A. & Martini, A. 1987. Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation. International Journal of Systematic Bacteriology, 37, 380-385. Grieve PA, et al.. Partial characterization of cheese-ripening proteinases produced by the yeast Kluyveromyces lactis. J. Dairy Res. 50: 469-480, 1983. Inchaurrondo VA, et al.. Yeast growth and -galactosidase production during aerobic batch cultures in lactose-limited synthetic medium. Process Biochem. 29: 47-54, 1994. Blondeau K, et al. Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture. Yeast 10: 1297-1303, 1994. Appl. Microbiol. Biotechnol. 40: 306-308, 1993. Biotechnol. Tech. 7: 609-614, 1993.

Physical Characteristics

  • Optimum TemperatureUnknown
  • Miniumum TemperatureUnknown
  • Maximum TemperatureUnknown

Cells

  • ShapeUnknown
  • Min Broth Breadth (µm)2
  • Max Broth Breadth (µm)4
  • Min Broth Length (µm)3
  • Max Broth Length (µm)7
  • Min Agar Breadth (µm)5
  • Max Agar Breadth (µm)7
  • Min Agar Length (µm)6
  • Max Agar Length (µm)8
  • ArrangementUnknown
  • Colour on AgarCream
  • Surface on AgarShiny
  • Texture on AgarSmooth
  • Deposit in BrothFlocculent
  • Ring in BrothAbsent
  • Ring ColourUnknown
  • Pellicle in BrothThin
  • Pellicle AppearanceMilky
  • Pellicle HabitatClimbing

Cell Division

  • BuddingMultipolar
  • FissionAbsent

Filamentous Growth

  • PseudomyceliumWell Formed
  • Pseudomycelium BranchIrregularly Branched
  • Pseudomycelium FormMycocandida
  • BlastosporesAbsent
  • Blastospore ShapeUnknown
  • Blastospore LocationUnknown
  • Blastospore HabitatUnknown
  • True MyceliumAbsent
  • Clamp ConnectionsAbsent

Asexual Spores

  • BallistosporesAbsent
  • AthrosporesAbsent
  • EndosporesAbsent
  • ChlamydosporesAbsent

Sexual Spores

  • AscosporesAbsent
  • Ascospore ShapeUnknown
  • Ascospore WallUnknown
  • Ascospores No Per AscusN/A
  • Ascus ShapeN/A
  • ConjugationAbsent
  • TeliosporesAbsent
  • Teliospore ShapeUnknown

Miscellaneous

  • AssayNo
  • Salt TolerantUnknown
  • KillerUnknown
  • PlasmidNo

Semi-Anaerobic Fermentation

  • Glucose+
  • GalactoseWeak/Latent
  • SucroseWeak/Latent
  • Maltose-
  • Cellobiose-
  • Trehalose-
  • LactoseWeak/Latent
  • Melibiose-
  • RaffinoseWeak/Latent
  • Melizitose-
  • Inulin-
  • Soluble Starch-
  • XyloseUnknown
  • AMD Glucoside-

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

  • NH4 2SO4+
  • KNO3-
  • Ethylamine+
  • Cadaverine+
  • LysineUnknown

Other

  • Vitamin Free Growth-
  • Cyclohex 100+
  • Cyclohex 1000-
  • Glucose Growth 50-
  • Glucose Growth 60Unknown
  • Lipolytic-
  • Acid Production-
  • Growth 37-
  • Growth 40Unknown
  • Arbutin Hydrolysis+
  • Urease Activity-
  • Starch Production-
  • Acid TolerantUnknown

Genomic Sequence Data

www.ebi.ac.uk/ena/browser/view/ERS13667410

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Norwich Research Park,
Norwich, NR4 7UQ, UK

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