NCYC 805

Saccharomyces cerevisiae

Taxon Synonyms

Saccharomyces cerevisiae

Equivalent Strain Designations

A364A; ATCC 22244

Depositor

J.F.T Spencer

Deposit Date

June 1974

Habitat

'General' mutant.

Details

Saccharomyces cerevisiae

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Key Information

Aerobic Utilisation and Growth

Glucose-
Galactose-
Sorbose-
Sucrose-
Maltose-
Cellobiose-
Trehalose-
Lactose-
Melibiose-
Raffinose-
Melezitose-
Inulun-
Soluble Starch-
Xylose-
L Arabinose-
D Arabinose-
Ribose-
Rhamnose-
Ethanol-
Glycerol-
Erythritol-
Ribitol-
Galactitol-
Mannitol-
Sorbitol-
AMD Glucoside-
Salicin-
Lactic Acid-
Succinic Acid-
Citric AcidUnknown
Inositol-
GluconolactoneUnknown
GlucosamineUnknown
MethanolUnknown
XylitolUnknown

Strain Information

Information
Mating type: a. Genotype: ade1 ade2 ura1 his7 lys2 tyr1 gal1. Assay of chlorhexidine diacetate (Hiom SJ, 1995). Produces killer toxin. Red cells are ade mutants, white are revertants, pink are partial revertants.
DepositorJ.F.T Spencer
Deposit NameSaccharomyces sp.
Month of depositJune
Deposit Year1974
Habitat'General' mutant.
Equivalent Strain DesignationsA364A; ATCC 22244
ReferenceJ. Mol. Biol., 1976, 105, 427-443. Wickner RB. Chromosomal and non-chromosomal mutations affecting thekiller character of Saccharomyces cerevisiae. Genetics 76: 423-432, 1974. Wickner RB, Leibowitz MJ . Two chromosomal genes required for killing expression in killer strains of Saccharomyces cerevisiae. Genetics 82: 429-442, 1976. Wickner RB. Deletion of mitochondrial DNA bypassing a chromosomal gene needed for maintenance of the killer plasmid of yeast. Genetics 87: 441-452, 1977. Wickner RB, Leibowitz MJ . Dominant chromosomal mutation bypassing chromosomal genes needed for killer RNA plasmid replication in yeast. Genetics 87: 453-469, 1977. Wickner RB. Twenty-six chromosomal genes needed to maintain the killer double-stranded RNA plasmid of Saccharomyces cerevisiae. Genetics 88: 419-425, 1978. Toh-E A , et al. Chromosomal superkiller mutants of Saccharomyces cerevisiae. J. Bacteriol. 136: 1002-1007, 1978. Wickner RB , Leibowitz MJ . Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast. J. Mol. Biol. 105: 427-443, 1976. Zinker S . P5/P5' the acidic ribosomal phosphoproteins from Saccharomyces cerevisiae. Biochim. Biophys. Acta 606: 76-82, 1980. J. Inst. Brew. 86: 78-80, 1980. Hiom SJ , et al. Uptake of 14C-chlorhexidine gluconate by Saccharomyces cerevisiae, Candida albicans and Candida glabrata. Lett. Appl. Microbiol. 21: 20-22, 1995. Hartwell LH . Synchronization of haploid yeast cell cycles, a prelude to conjugation. Exp. Cell Res. 76: 111-117, 1973. Hiom SJ , et al. X-ray microanalysis of chlorhexidine-treated cells of Saccharomyces cerevisiae. Lett. Appl. Microbiol. 20: 353-356, 1995. Hartwell LH . Macromolecule synthesis in temperature-sensitive mutants of yeast. J. Bacteriol. 93: 1662-1670, 1967. Nelson RG , Fangman WL . Nucleosome organization of the yeast 2-micrometer DNA plasmid: a eukaryotic minichromosome. Proc. Natl. Acad. Sci. USA 76: 6515-6519, 1979. Shulman RW , et al. Noncoordinated transcription in the absence of protein synthesis in yeast. J. Biol. Chem. 252: 1344-1349, 1977. Hartwell LH , McLaughlin CS . Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J. Bacteriol. 96: 1664-1671, 1968.

Physical Characteristics

Optimum TemperatureUnknown
Miniumum TemperatureUnknown
Maximum TemperatureUnknown

Cells

ShapeUnknown
Min Broth Breadth (µm)2
Max Broth Breadth (µm)4
Min Broth Length (µm)3
Max Broth Length (µm)6
Min Agar Breadth (µm)3
Max Agar Breadth (µm)5
Min Agar Length (µm)3
Max Agar Length (µm)5
ArrangementPairs
Colour on AgarRed
Surface on AgarDull
Texture on AgarSmooth
Deposit in BrothNon-Flocculent
Ring in BrothAbsent
Ring ColourUnknown
Pellicle in BrothAbsent
Pellicle AppearanceUnknown
Pellicle HabitatUnknown

Cell Division

BuddingMultipolar
FissionAbsent

Filamentous Growth

PseudomyceliumAbsent
Pseudomycelium BranchUnknown
Pseudomycelium FormN/A
BlastosporesUnknown
Blastospore ShapeUnknown
Blastospore LocationUnknown
Blastospore HabitatUnknown
True MyceliumAbsent
Clamp ConnectionsAbsent

Asexual Spores

BallistosporesAbsent
AthrosporesAbsent
EndosporesAbsent
ChlamydosporesAbsent

Sexual Spores

AscosporesAbsent
Ascospore ShapeUnknown
Ascospore WallUnknown
Ascospores No Per AscusN/A
Ascus ShapeN/A
ConjugationAbsent
TeliosporesAbsent
Teliospore ShapeUnknown

Miscellaneous

AssayUnknown
Salt TolerantUnknown
KillerUnknown
PlasmidUnknown

Semi-Anaerobic Fermentation

Glucose+
Galactose-
Sucrose+
Maltose-
CellobioseUnknown
TrehaloseUnknown
Lactose-
Melibiose-
Raffinose-
MelizitoseUnknown
InulinUnknown
Soluble Starch-
XyloseUnknown
AMD GlucosideUnknown

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4-
KNO3-
EthylamineUnknown
CadaverineUnknown
LysineUnknown

Other

Vitamin Free Growth-
Cyclohex 100-
Cyclohex 1000-
Glucose Growth 50Unknown
Glucose Growth 60-
Lipolytic-
Acid Production-
Growth 37+
Growth 40Unknown
Arbutin Hydrolysis-
Urease ActivityUnknown
Starch ProductionUnknown
Acid TolerantUnknown

Genomic Sequence Data

No data