NCYC 1429

Kluyveromyces marxianus

Taxon Synonyms

Kluyveromyces marxianus

Equivalent Strain Designations

ATCC 12424, NRRL Y-610, VTT C-83140.

Depositor

ATCC

Deposit Date

August 1982

Habitat

Unknown

Details

Kluyveromyces marxianus

A licence fee may be applied to products purchased for commercial use. Find out more information.

Customer Type Supplied As Recovery Media

Add to Basket

Key Information

Aerobic Utilisation and Growth

Glucose +
Galactose +
Sorbose -
Sucrose +
Maltose -
Cellobiose +
Trehalose -
Lactose +
Melibiose -
Raffinose +
Melezitose -
Inulun +
Soluble Starch -
Xylose +
L Arabinose +
D Arabinose -
RiboseWeak/Latent
Rhamnose -
Ethanol +
Glycerol +
Erythritol -
RibitolWeak/Latent
Galactitol -
MannitolWeak/Latent
SorbitolWeak/Latent
AMD Glucoside -
Salicin +
Lactic Acid +
Succinic Acid +
Citric Acid -
Inositol -
Gluconolactone -
Glucosamine -
Methanol -
XylitolUnknown

Strain Information

Information
Produces inulase (beta-fructofuranosidase), an alternative to invertase for use in confectionary industry. Produces NAD-dependent alcohol dehydrogenase. Produces acetoin dehydrogenase (diacetyl reductase). Produces beta-fructofuranosidase (beta-fructosidase). Produces inulase. Produces inulinase (endoinulinase). Produces lactase. Used as a transformation host.
DepositorATCC
Deposit NameKluyveromyces fragilis
Month of depositAugust
Deposit Year1982
HabitatUnknown
Equivalent Strain DesignationsATCC 12424, NRRL Y-610, VTT C-83140.
ReferenceWorkman WE , Day DF . Purification and properties of the beta-fructofuranosidase from Kluyveromyces fragilis. FEBS Lett. 160: 16-20, 1983. Workman WE , Day DF . Enzymatic hydrolysis of inulin to fructose by glutaraldehyde fixed yeast cells. Biotechnol. Bioeng. 26: 905-910, 1984. Schwarz JG , Hang YD . Study of acetoin reductase from Kluyveromyces marxianus. Lett. Appl. Microbiol. 16: 14-16, 1993. Wickerham LJ , Burton KA . Hydridization studies involving Saccharomyces lactis and Zygosaccharomyces ashbyi. J. Bacteriol. 71: 290-302, 1956. Biotechnol. Lett. 7: 179-184, 1985. Iborra F . High efficiency transformation of Kluyveromyces marxianus by a replicative plasmid. Curr. Genet. 24: 181-183, 1993. Laloux O , et al. Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424. FEBS Lett. 289: 64-68, 1991. Groot Wassink JW , Fleming SE . Non-specific beta-fructofuranosidase (inulase) from Kluyveromyces fragilis: batch and continuous fermentation, simple recovery method, and some industrial properties. Enzyme Microb. Technol. 2: 45-53, 1980. Hewitt GM , Grootwassink JW . Simultaneous production of inulase and lactase in batch and continuous cultures of Kluyveromyces fragilis. Enzyme Microb. Technol. 6: 263-270, 1984. Schwarz JG , Hang YD . Kluyveromyces marxianus: a potential source of diacetyl reductase. World J. Microbiol. Biotechnol. 10: 385-387, 1994. Sanfacon R , et al. Alcohol dehydrogenase activity in the yeast Saccharomyces fragilis. Can. J. Microbiol. 18: 35-44, 1972.

Physical Characteristics

Optimum Temperature42
Miniumum Temperature12
Maximum Temperature47

Cells

ShapeLong-oval
Min Broth Breadth (µm)2
Max Broth Breadth (µm)5
Min Broth Length (µm)5
Max Broth Length (µm)15
Min Agar Breadth (µm)3
Max Agar Breadth (µm)7
Min Agar Length (µm)4
Max Agar Length (µm)12
ArrangementSingle
Colour on AgarCream
Surface on AgarShiny
Texture on AgarSmooth
Deposit in BrothNon-Flocculent
Ring in BrothAbsent
Ring Colour N/A
Pellicle in BrothAbsent
Pellicle AppearanceN/A
Pellicle HabitatN/A

Cell Division

BuddingMultipolar
FissionAbsent

Filamentous Growth

PseudomyceliumWell formed
Pseudomycelium BranchIrregularly branched
Pseudomycelium FormUnknown
BlastosporesAbsent
Blastospore ShapeN/A
Blastospore LocationN/A
Blastospore HabitatN/A
True MyceliumAbsent
Clamp ConnectionsAbsent

Asexual Spores

BallistosporesAbsent
AthrosporesAbsent
EndosporesAbsent
ChlamydosporesAbsent

Sexual Spores

AscosporesPresent
Ascospore ShapeReniform
Ascospore WallUnknown
Ascospores No Per Ascus02-Mar
Ascus ShapeOval
ConjugationAbsent
TeliosporesAbsent
Teliospore ShapeN/A

Miscellaneous

AssayUnknown
Salt TolerantUnknown
KillerUnknown
PlasmidUnknown

Semi-Anaerobic Fermentation

Glucose +
Galactose +
Sucrose +
Maltose -
Cellobiose -
Trehalose -
Lactose +
Melibiose -
Raffinose +
Melizitose -
Inulin +
Soluble Starch -
XyloseUnknown
AMD Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine +
CadaverineUnknown
LysineUnknown

Other

Vitamin Free Growth -
Cyclohex 100 +
Cyclohex 1000Unknown
Glucose Growth 50 -
Glucose Growth 60Unknown
LipolyticUnknown
Acid Production -
Growth 37 +
Growth 40 +
Arbutin Hydrolysis +
Urease Activity -
Starch Production -
Acid TolerantUnknown

Genomic Sequence Data

No data