NCYC 175

Komagataella pastoris

Pre 2011 Name

Pichia pastoris

Equivalent Strain Designations

ATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.

Depositor

A. Guilliermond

Deposit Date

Unknown 1925

Habitat

Exudate of Chestnut tree, near Lyon, France.
Komagataella pastoris

Details

Komagataella pastoris

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Key Information

Aerobic Utilisation and Growth

Glucose +
Galactose -
Sorbose -
Sucrose -
Maltose -
Cellobiose -
Trehalose +
Lactose -
Melibiose -
Raffinose -
Melezitose -
Inulun -
Soluble Starch -
Xylose -
L Arabinose -
D Arabinose -
Ribose -
Rhamnose +
Ethanol +
Glycerol +
Erythritol -
Ribitol -
Galactitol -
Mannitol +
SorbitolWeak/Latent
AMD Glucoside -
Salicin -
Lactic AcidWeak/Latent
Succinic Acid +
Citric Acid +
Inositol -
Gluconolactone -
Glucosamine -
Methanol +
Xylitol -

Strain Information

Information
Production of yeast protein from hydrocarbons (US Patent 4,226,939). Produces natural flavor aldehydes (Murray WD, 1989). Converts ethanol to acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Degrades ethyl alcohol (ethanol) (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Degrades hydrogen peroxide (Duff SJB, Murray WD, 1990). Produces acetaldehyde (Duff SJ, Murray WD, 1988a; Duff SJ, Murray WD, 1988b; Duff SJ, Murray WD, 1988c; Appl. Microbiol. Biotechnol. 36: 82-86, 1991; Enzyme Microb. Technol. 11: 770-775, 1989). Produces alcohol oxidase (Murray WD, et al, 1989; Murray WD, et al, 1990; Agric. Biol. Chem. 54: 1879-1882, 1990).
DepositorA. Guilliermond
Deposit NameSaccharomyces pastori / Zygosaccharomyces pastori
Month of depositUnknown
Deposit Year1925
HabitatExudate of Chestnut tree, near Lyon, France.
Equivalent Strain DesignationsATCC 28485, CCRC 21531, CBS 704, JCM 3650, NCTC 2058, NRRL Y-1603.
ReferenceGuilliermond, CR.Hebd. Séances Mem.Soc.Biol. 82: 466-470, 1919. Nakase & Komagata, J.Gen.Appl.Microbiol. 16: 511-521, 1970 (GC). Bicknell & Douglas, J.Bacteriol. 101: 505, 1970 (GC). Yamada et al., J.Gen.Appl.Microbiol. 19: 189-208, 1973 (CoQ). Duff SJ, Murray WD. Production and application of methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 31: 44-49, 1988a. Duff SJ, Murray WD. Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde. Biotechnol. Bioeng. 31: 790-795, 1988b. Duff SJ, Murray WD. Production of flavor aldehydes using nongrowing whole cells of Pichia pastoris. Ann. N.Y. Acad. Sci. 542: 428-433, 1988c. Murray WD, et al. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32: 95-100, 1989. Duff SJ, Murray WD. Oxidation of benzyl alcohol by whole cells of Pichia pastoris and by alcohol oxidase in aqueous and nonaqueous reaction media. Biotechnol. Bioeng. 34: 153-159, 1989. Murray WD, et al. Catabolite inactivation in the methylotrophic yeast Pichia pastoris. Appl. Environ. Microbiol. 56: 2378-2383, 1990. Wegner EH. Treatment of make-up water for use in a fermentation process for growth of yeast cells requiring growth factors. US Patent 4,226,939 dated Oct 7 1980 Murray WD, et al. Production of natural flavor aldehydes from natural source primary alcohols C2-C7. US Patent 4,871,669 dated Oct 3 1989 Phaff HJ. A proposal for amendment of the diagnosis of the genus Pichia Hansen. Antonie van Leeuwenhoek 22: 113-116, 1956. Appl. Microbiol. Biotechnol. 36: 82-86, 1991. Yamada Y, et al. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci. Biotechnol. Biochem. 59: 439-444, 1995. Enzyme Microb. Technol. 11: 770-775, 1989. Agric. Biol. Chem. 54: 1879-1882, 1990. Duff SJB, Murray WD. Metabolism of hydrogen peroxide by Pichia pastoris. Agric. Biol. Chem. 54: 1967-1974, 1990.

Physical Characteristics

Optimum TemperatureUnknown
Miniumum TemperatureUnknown
Maximum TemperatureUnknown

Cells

ShapeOval
Min Broth Breadth (µm)3
Max Broth Breadth (µm)5
Min Broth Length (µm)3
Max Broth Length (µm)6
Min Agar Breadth (µm)2
Max Agar Breadth (µm)5
Min Agar Length (µm)3
Max Agar Length (µm)6
ArrangementPairs
Colour on AgarCream
Surface on AgarShiny
Texture on AgarSmooth
Deposit in BrothNon-Flocculent
Ring in BrothIncomplete
Ring ColourWhite
Pellicle in BrothAbsent
Pellicle AppearanceN/A
Pellicle HabitatN/A

Cell Division

BuddingMultipolar
FissionAbsent

Filamentous Growth

PseudomyceliumWell formed
Pseudomycelium BranchIrregularly branched
Pseudomycelium FormMycocandida
BlastosporesAbsent
Blastospore ShapeN/A
Blastospore LocationN/A
Blastospore HabitatN/A
True MyceliumAbsent
Clamp ConnectionsAbsent

Asexual Spores

BallistosporesAbsent
AthrosporesAbsent
EndosporesAbsent
ChlamydosporesAbsent

Sexual Spores

AscosporesPresent
Ascospore ShapeHat
Ascospore WallUnknown
Ascospores No Per Ascus4
Ascus ShapeUnknown
ConjugationPresent
TeliosporesAbsent
Teliospore ShapeN/A

Miscellaneous

AssayUnknown
Salt TolerantUnknown
KillerNo
PlasmidNo

Semi-Anaerobic Fermentation

Glucose +
Galactose -
Sucrose -
Maltose -
Cellobiose -
Trehalose -
Lactose -
Melibiose -
Raffinose -
Melizitose -
Inulin -
Soluble Starch -
XyloseUnknown
AMD Glucoside -

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4 +
KNO3 -
Ethylamine +
Cadaverine +
LysineUnknown

Other

Vitamin Free GrowthWeak/Latent
Cyclohex 100 +
Cyclohex 1000 +
Glucose Growth 50 -
Glucose Growth 60 -
Lipolytic -
Acid ProductionWeak/Latent
Growth 37 -
Growth 40Unknown
Arbutin Hydrolysis -
Urease Activity -
Starch Production -
Acid TolerantUnknown

Genomic Sequence Data

No data