NCYC 87

Aerobic Utilisation and Growth

Glucose+
Galactose+
Sorbose-
Sucrose+
Maltose+
Cellobiose-
Trehalose+
Lactose-
Melibiose-
Raffinose+
MelezitoseWeak/Latent
Inulun-
Soluble Starch-
Xylose-
L Arabinose-
D Arabinose-
Ribose-
Rhamnose-
Ethanol+
Glycerol-
Erythritol-
Ribitol-
GalactitolUnknown
Mannitol-
Sorbitol-
AMD Glucoside+
Salicin-
Lactic AcidWeak/Latent
Succinic Acid-
Citric Acid-
Inositol-
Gluconolactone-
Glucosamine-
Methanol-
Xylitol-

Strain Information

Information
Applications: Produces ethanol from saccharified whole corn mash (Ziffer J & Iosif MI , 1982). Assay of amphotericin (British Pharmacopoeia Appendix XIV A, 2003). Assay of amphotericin B (fungizone) (Pharmacopeia; USP USP28-NF23, 2005). Assay of anisomycin (Bowman FW, 1957). Assay of antifongine (Hagemann G, 1962). Assay of candicidin (U.S. Pharmacopeia; USP USP28-NF23, 2005). Assay of natamycin (pimaricin; tennecetin). Assay of nystatin (fungicidin) (British Pharmacopoeia Appendix XIV A, 2003; European Pharmacopoeia EP 2.7.2, 1997; AOAC International. 957.23.; Bowman FW, 1957; Platt TB & Itkin AG, 1974). Media testing (International Organization for Standardization/ANSI; ISO ISO 11133-2:2003). Produces arginase; British Standard DD CEN ISO/TS 11133:2003). Produces ethyl alcohol (ethanol) (Ziffer J & Iosif MI, 1982). Produces histone deacetylase (Sanchez del Pino MM, 1994). Produces nicotinic acid (niacin) (Tseng MM & Phillips CR, 1982). Sterility testing (Bowman FW, 1971). Susceptibility testing (Brenner DJ, 1994; Pfaller MA, 1994; Deutsches Institut fur Normung; DIN DIN 58940-84, 2002) Testing disinfectants Testing fungicides (British Standard BS EN 1650:1998; British Standard BS EN 12353:2006; British Standard BS EN 13697:2001; British Standard BS EN 14885:2006). API Q C culture (bioMerieux Vitek) used in Commercial identification kits.
DepositorATCC
Deposit NameSaccharomyces cerevisiae
Month of depositMay
Deposit Year1947
HabitatDistillery yeast.
Equivalent Strain DesignationsATCC 9763, CBS 2978, CCRC 20822, DSM 1333, NCTC 7239, NCTC 10716, NRRL Y-567.
ReferenceZiffer J & Iosif MI. Temperature effects in ethanol fermentation high corn media. Biotechnol. Lett. 4: 809-814, 1982. Microbial assay for antibiotics. Tokyo, Japan: Japanese Pharmacopoeia; JP JP14e.part I.34. British Pharmacopoeia Commission. Biological assay of antibiotics. London, UK: British Pharmacopoeia Commission; British Pharmacopoeia Appendix XIV A, 2003. U.S. Pharmacopeia. General Chapters; <81> ANTIBIOTICS-MICROBIAL ASSAYS. Rockville, MD: U.S. Pharmacopeia; USP USP28-NF23, 2005. Bowman FW. Test organisms for antibiotic microbial assays. Antibiot. Chemother. 7: 639-640, 1957. Hagemann G, et al.. Fungicidal compound and process of making same. US Patent 3,052,605 dated Sep 4 1962. U.S. Pharmacopeia. General Chapters; <81> ANTIBIOTICS-MICROBIAL ASSAYS. Rockville, MD: U.S. Pharmacopeia; USP USP28-NF23, 2005. British Pharmacopoeia Commission. Biological assay of antibiotics. London, UK: British Pharmacopoeia Commission; British Pharmacopoeia Appendix XIV A, 2003. European Pharmacopoeia Commission. Microbiological assay of antibiotics. Strasbourg, France: European Pharmacopoeia Commission; European Pharmacopoeia EP 2.7.2, 1997. AOAC International. Antibiotics in feeds, microbiological methods. Gaithersburg, MD: AOAC International; AOACOfficial Methods of Analysis of the AOAC International 957.23. Bowman FW . Test organisms for antibiotic microbial assays. Antibiot. Chemother. 7: 639-640, 1957. Platt TB & Itkin AG . Microbiological assay of nystatin in feeds. J. Assoc. Off. Anal. Chem. 57: 536-540, 1974. Microbiology of food and animal feeding stuffs-Guidelines on preparation and production of culture media- Part 2: Practical guidelines on performance testing of culture media. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 11133-2:2003. Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK: British Standards Institution; British Standard DD CEN ISO/TS 11133:2003. Ziffer J & Iosif MI. Temperature effects in ethanol fermentation high corn media. Biotechnol. Lett. 4: 809-814, 1982. Sanchez del Pino MM et al. Properties of the yeast nuclear histone deacetylase. Biochem. J. 303: 723-729, 1994. Tseng MM & Phillips CR. The kinetics of yeast growth and nicotinic acid production. Biotechnol. Bioeng. 24: 1319-1325, 1982. Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971. Brenner DJ , et al. Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates. J. Clin. Microbiol. 32: 1625-1628, 1994. Pfaller MA , et al. Selection of candidate quality control isolates and tentative quality control ranges for in vitro susceptibility testing of yeast isolates by National Committee for Clinical Laboratory Standards proposed standard methods. J. Clin. Microbiol. 32: 1650-1653, 1994. Medical microbiology-Susceptibility testing of microbial pathogens to antimicrobial agents - Part 84: Microdilution; Special requirements for testing of fungi against antifungal agents. Berlin, Germany: Deutsches Institut fur Normung; DIN DIN 58940-84: 2002, 2002. Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1). London, UK: British Standards Institution; British Standard BS EN 1650:1998. Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK: British Standards Institution; British Standard BS EN 12353:2006. Chemical disinfectants and antiseptics - Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas --- Test method and requirements without mechanical action (phase 2/step 2). London, UK: British Standards Institution; British Standard BS EN 13697:2001. Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK: British Standards Institution; British Standard BS EN 14885:2006. US Fed.Regulations 27: 13047 & 1322, 1962 and 28: 3823, 1963. Clemons et al., J.Infect.Dis. 169: 859-867, 1994. McCusker, Infect.Immun. 62: 5447-5455, 1994. British Pharmacopoeia, 1980, 2, A123. US Pharmacopoeia, 1984, 20, %th suppl., 1098-1102. J. Biol. Chem., 1980, 255, 6387 (studies on invertase).

Physical Characteristics

Optimum TemperatureUnknown
Miniumum TemperatureUnknown
Maximum TemperatureUnknown

Cells

ShapeUnknown
Min Broth Breadth (µm)4
Max Broth Breadth (µm)7
Min Broth Length (µm)4
Max Broth Length (µm)12
Min Agar Breadth (µm)3
Max Agar Breadth (µm)9
Min Agar Length (µm)5
Max Agar Length (µm)13
ArrangementSingle
Colour on AgarCream
Surface on AgarDull
Texture on AgarWrinkled
Deposit in BrothNon-Flocculent
Ring in BrothComplete
Ring ColourWhite
Pellicle in BrothThick
Pellicle AppearanceIslands
Pellicle HabitatClimbing

Cell Division

BuddingMultipolar
FissionAbsent

Filamentous Growth

PseudomyceliumWell Formed
Pseudomycelium BranchUnknown
Pseudomycelium FormUnknown
BlastosporesAbsent
Blastospore ShapeUnknown
Blastospore LocationUnknown
Blastospore HabitatUnknown
True MyceliumAbsent
Clamp ConnectionsAbsent

Asexual Spores

BallistosporesAbsent
AthrosporesAbsent
EndosporesAbsent
ChlamydosporesAbsent

Sexual Spores

AscosporesPresent
Ascospore ShapeRound
Ascospore WallSmooth
Ascospores No Per Ascus02-Apr
Ascus ShapeUnknown
ConjugationAbsent
TeliosporesAbsent
Teliospore ShapeUnknown

Miscellaneous

AssayYes
Salt Tolerant -
KillerUnknown
PlasmidYes

Semi-Anaerobic Fermentation

Glucose+
Galactose+
Sucrose+
Maltose+
Cellobiose-
Trehalose-
Lactose-
Melibiose-
Raffinose-
Melizitose-
Inulin-
Soluble StarchUnknown
XyloseUnknown
AMD Glucoside-

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

NH4 2SO4+
KNO3-
Ethylamine-
Cadaverine-
LysineUnknown

Other

Vitamin Free Growth-
Cyclohex 100-
Cyclohex 1000-
Glucose Growth 50-
Glucose Growth 60-
Lipolytic-
Acid ProductionWeak/Latent
Growth 37+
Growth 40Unknown
Arbutin Hydrolysis-
Urease Activity-
Starch Production-
Acid Tolerant-

Brewing Information

DescriptionUnknown
DepositUnknown
HeadUnknown
AttenuationUnknown
ClarityUnknown
Fermentation RateUnknown
FlocculenceUnknown

Genomic Sequence Data

www.ebi.ac.uk/ena/browser/view/ERR5285567

Saccharomyces cerevisiae

Taxon Synonyms

Equivalent Strain Designations

Depositor

Deposit Date

Habitat

Details

Key Information

Aerobic Utilisation and Growth

Strain Information

Physical Characteristics

Cells

Cell Division

Filamentous Growth

Asexual Spores

Sexual Spores

Miscellaneous

Semi-Anaerobic Fermentation

Aerobic Utilisation and Growth - Sole Sources of Nitrogen

Other

Brewing Information

Genomic Sequence Data